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Chronic fatigue syndrome (myalgic encephalomyelitis)

Chronic fatigue syndrome (CFS) is the most common name given to a variably debilitating disorder or disorders generally defined by persistent fatigue unrelated to exertion, not substantially relieved by rest and accompanied by the presence of other specific symptoms for a minimum of six months. Other symptoms include musculoskeletal pain, sleep disturbance, impaired concentration, and headaches.

Almost every aspect of CFS is the subject of disagreement and uncertainty1 – even diagnosis. There are no characteristic laboratory abnormalities to diagnose CFS,2 so testing is used to rule out other potential causes for symptoms. When symptoms are attributable to certain other conditions, the diagnosis of CFS is excluded.

The absence of well-defined biomarkers coupled with an ability to point to a single somatic cause (e.g. an infectious process) has had a devastating effect on the way patients with CFS/ME are diagnosed, treated and regarded. Even though CFS/ME is now widely regarded as a “real” disease, the most popular explanation for this illness remains that it represents an extreme form of “medically unexplained illnesses.” As such, many psychiatrists have speculated that the condition is the product of maladaptive behaviors, failure to self-regulate, or any of dozens of others profoundly psychological or behavioral causes.3

An infectious etiology of chronic fatigue syndrome has long been suspected. Today, scientific evidence provides a growing, if not conclusive, link between infectious agents and CFS. For example, a 2011 study detected hundreds of unique proteins in the cerebrospinal fluid of patients with CFS as compared to healthy controls.

Presentation at the International Autoimmunity congress on the treatment of CFS with the Marshall protocol:

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Contested name

Chronic fatigue syndrome (CFS) is the name currently used by the majority of the medical and scientific community to describe a condition or set of conditions characterized by fatigue and other symptoms. The term is contested, mostly by patients and patient advocacy groups, but also by some doctors. Several of the more common alternative names used to describe what most believe to be the same condition or subtypes include:

  • myalgic encephalomyelitis (ME)
  • chronic fatigue immune dysfunction syndrome (CFIDS)
  • post-viral syndrome
  • post-viral fatigue syndrome (PVFS)
  • Gulf war syndrome

Hillary Johnson offers this partial history of the origin of the term “chronic fatigue syndrome”:

The illness became famous after an outbreak in 1984 around Lake Tahoe, in Nevada. Several hundred patients developed flu-like symptoms like fever, sore throat and headaches that led to neurological problems, including severe memory loss and inability to understand conversation. Most of them were infected with several viruses at once, including cytomegalovirus, Epstein-Barr and human herpesvirus 6. Their doctors were stumped. The Centers for Disease Control and Prevention, the nation’s presumed bulwark against emerging infectious diseases, dismissed the epidemic and said the Tahoe doctors “had worked themselves into a frenzy.” The sufferers, a C.D.C. investigator told me at the time, were “not normal Americans.”

When, by 1987, the supposed hysteria failed to evaporate and indeed continued erupting in other parts the country, the health agency orchestrated a jocular referendum by mail among a handful of academics to come up with a name for it. The group settled on “chronic fatigue syndrome” — the use of “syndrome” rather than “disease” suggested a psychiatric rather than physical origin and would thus discourage public panic and prevent insurers from having to make “chronic disbursements,” as one of the academics joked.

An 11th-hour plea by a nascent patient organization to call the disease by the scientific name used in Britain, myalgic encephalomyelitis, was rejected by the C.D.C. as “overly complicated and too confusing for many nonmedical persons.”

Had the agency done nothing in response to this epidemic, patients would now be better off. The name functioned as a kind of social punishment. Patients were branded malingerers by families, friends, journalists and insurance companies, and were denied medical care. (It’s no coincidence that suicide is among the three leading causes of death among sufferers.) Soon the malady came to be widely considered a personality disorder or something that sufferers brought upon themselves. A recent study financed by the C.D.C. suggested that childhood trauma or sexual abuse, combined with a genetic inability to handle stress, is a key risk factor for chronic fatigue syndrome.

Hillary Johnson, New York Times

However, CFS is a serious disease. The gentleness of its name barely suggests how debilitating the affliction is, and the use of the term “fatigue” diminishes the legitimacy of the illness. According to a survey of medical trainees at a school in the United States, a condition described as chronic fatigue syndrome may be considered less serious than a condition described as myalgic encephalopathy.4

Diagnosis and testing

In the U.S., the most commonly used diagnostic criteria and definition of CFS/ME for research and clinical purposes were published by the United States Centers for Disease Control and Prevention (CDC).5 The Centers for Disease Control definition of CFS requires the following two criteria be fulfilled:

  1. A new onset (not lifelong) of unexplained, persistent fatigue unrelated to exertion and not substantially relieved by rest, that causes a significant reduction in previous activity levels.
  2. Four or more of the following symptoms that last six months or longer: impaired memory or concentration; post-exertional malaise, where physical or mental exertions bring on “extreme, prolonged exhaustion and sickness”; unrefreshing sleep; muscle pain (myalgia); pain in multiple joints (arthralgia); headaches of a new kind or greater severity; sore throat, frequent or recurring; tender lymph nodes (cervical or axillary).

There are no characteristic laboratory abnormalities to diagnose CFS/ME,6 so testing is used to rule out other potential causes for symptoms.7 When symptoms are attributable to certain other conditions, the diagnosis of CFS is excluded.

The absence of clear laboratory markers affects not only how CFS is diagnosed, but whether or not it is viewed as fundamentally psychological illness, and how it is treated.

Symptoms

Fatigue

Related articles: Fatigue, Stimulants

Patients with CFS may have levels of fatigue comparable to patients with late-stage AIDS.

My HIV patients for the most part are hale and hearty thanks to three decades of intense and excellent research and billions of dollars invested. Many of my CFS patients, on the other hand, are terribly ill and unable to work or participate in the care of their families. I split my clinical time between the two illnesses, and I can tell you if I had to choose between the two illnesses (in 2009) I would rather have HIV. But CFS, which impacts a million people in the United States alone, has had a small fraction of the research dollars directed towards it.

Nancy Klimas, M.D., New York Times

The systematic use of stimulants to manage symptoms of fatigue is not recommended.

Insomnia and poor sleep

The inability to sleep or sleep deeply is a common symptom of chronic inflammatory disease and can be exacerbated during periods of immunopathology. Restful sleep can help a patient to cope with other symptoms. It is not necessary to get all one's restful sleep at night. In fact, if a patient can, sleeping during the day may give him or her enough energy to accomplish any responsibilities for the day.

Cognitive dysfunction

Main article: Cognitive dysfunction

Cognitive dysfunction (also known as brain fog) is the loss of intellectual functions such as thinking, remembering, and reasoning of sufficient severity to interfere with daily functioning. Patients with cognitive dysfunction have trouble with verbal recall, basic arithmetic, and concentration.

According to the Marshall Pathogenesis, cognitive dysfunction is caused by microbes. More severe forms of cognitive dysfunction are seen in diseases such as Alzheimer's, diseases for which there is strong evidence of a bacterial etiology. Often associated with chronic fatigue syndrome,8 cognitive dysfunction is also seen in patients with multiple sclerosis,9 depression,10 fibromyalgia,11 and dozens of others diseases.

Like all symptoms of inflammatory disease, cognitive dysfunction may temporarily increase during periods of immunopathology. Cognitive dysfunction can be managed using the generic strategies for managing immunopathology, and should resolve over the course of the Marshall Protocol (MP).

Some studies seem to suggest that sick women experience cognitive dysfunction more frequently and more severely than their male counterparts.12 13

Pain

Pain is a symptom of Th1 disease and can be exacerbated by immunopathology. Marshall Protocol (MP) patients should always use 40mg of olmesartan (Benicar) every four hours before resorting to pain medications. There are also other strategies for managing pain.

When the usual strategies for managing immunopathology are not enough to control pain, Marshall Protocol (MP) patients rely upon pain medications. Except for corticosteroids, there is no pain medication contraindicated specifically because a patient is on the Marshall Protocol. Opioids are the preferred method of dealing with extreme pain in the MP cohort.

The effects of pain medications drugs on the immune system are not known. At present, it can't be said to what extent these drugs interfere with immunopathology. While it's strongly possible that some pain medications might temper immune system activity, patients taking them have made progress while taking them. As long as patients feel they're still experiencing immunopathology and that the medications are taken for reasons of necessity, pain medications are not contraindicated.

In conjunction with their physician or a pain management specialist, patients may want to experiment with different pain killers to see if any of them seem to lower not just pain but other disease symptoms. If they feel a particular pain medication is lowering immunopathology they are able to tolerate, they may want to ask their physician to change to a medication that does not elicit such an effect.

Exercise intolerance

For Marshall Protocol (MP) patients, the choice to exert oneself through physical activity or exercise should be made in the light of the stage of one's disease and the effect that extra activity has on the immune system. In different patients under different circumstances, exercise is capable of either suppressing or increasing the immune response.

MP patients who can perform their activities of daily living while recovering on the MP are as fit as they need to be. Their focus should be in regaining their health with the MP. With improved health will come improved abilty to exercise and increase endurance.

Cardiac problems

Related article: Managing cardiac symptoms
Related article: Cardiovascular diseases

When in doubt, seek medical attention immediately. Call 911 (unlock your door if you are alone). Chew one 325mg uncoated aspirin. Lie down so your heart doesn't have to work so hard. If you think you might pass out, try forcing yourself to cough deeply. (It changes the pressure in your chest and can have the same effect as the thump given in CPR.)

The innate immune system response to microbes results in low-grade, often subclinical inflammation and chronic disease. Its effects can be seen in any part of the body including the heart. Treating chronic infections with the Marshall Protocol (MP) unavoidably results in increased inflammation. While a severe cardiac immunopathological reaction is rare, it has the potential to be life-threatening. Therefore, health care providers are cautioned to be on the alert for cardiac symptoms in all their patients. Also, patients with risk factors should know when seek medical attention.

Physicians and patients anticipating strong cardiac immunopathology should also review these documents:

Respiratory problems

Patients at risk for respiratory problems should ask their physicians for a prescription for supplemental oxygen.

A variety of strategies that do not involve medication are available for patients who have uncomfortable respiratory symptoms including breathing exercises, getting more fluids, rest, and others. Also, patients have reported relief taking guaifenesin, using bronchodilator inhalers (steroid inhalers are contraindicated), and nebulizers. Supplemental oxygen may be useful or necessary in some cases even though it may be needed only for a few hours a day for a few months.

While it is certainly possible to contract an acute respiratory infection while on the Marshall Protocol, many symptoms of immunopathology mimic those of an acute respiratory infection. Early recognition and effective management of immunopathology are very important when a patient has respiratory symptoms. Any symptom that correlates with MP therapy may be due to immunopathology.

Emergency medical personnel should know that a patient is on the MP. The article Notice for health care providers provides information that emergency medical personnel need to know.

Evidence of infectious cause

Today scientific evidence provides a growing link between infectious agents and Chronic Fatigue Syndrome.

History of conflicting results

An infectious etiology of Chronic Fatigue Syndrome has long been suspected and investigated, often with inconsistent results.14 15 The debate over whether XMRV, EBV, or any other single pathogen causes CFS reflects an over-reliance on the antiquated model of disease set forth by Koch's postulates – namely that a pathogen must be: found in all cases of the disease examined, prepared and maintained in a pure culture, capable of producing the original infection, even after several generations in culture, and retrievable from an inoculated animal and cultured again. For all their lingering influence, Koch's postulates never anticipated the era of the human metagenome in which thousands of difficult or impossible-to-culture species of bacteria – rather than just one – contribute to a single disease state.

Proteins from the cerebrospinal fluid of patients with Chronic Fatigue Syndrome (CFS), Neurologic Post Treatment Lyme Syndrome (nPTLS), and healthy controls. This analysis strongly suggests a biological component of these diseases as well as a large role for microbes, which have profound effects on the human proteome.

Different proteins in cerebrospinal fluid

In a 2011 study appearing in PLoS One, Schutzer and colleagues analyzed spinal fluid samples from three groups — 11 healthy people, 43 diagnosed with chronic fatigue syndrome and 25 previously treated for Lyme disease but who were still experiencing cognitive problems and fatigue.16 Analysis of the fluid samples detected more than 2,600 proteins in each group. Most of the proteins appeared in all three groups. But 692 proteins turned up only in the Lyme patients and 738 others showed up only in the chronic fatigue group.

On its most basic level, this study offers strong evidence of a biological component for Lyme and CFS. The presence of so many unique proteins suggests that these are proteins are either created directly by microbes or are the results of microbes acting on the human genome.

In the aftermath of the study, several commentators have noted that this work could be built upon to more definitely distinguish Lyme from CFS. However, it is interesting to note that a significant number of proteins are common to both CFS and Lyme. The reductionist approach, in which a disease state is defined by a single or even a handful of proteins may not fully account for the complexity of human microbiome and the nature of the disease process.

EBV

In the late 1980s and early 1990s, EBV became the favored explanation for chronic fatigue syndrome. It was noted that people with chronic exhaustion had EBV, although it was also noted EBV was present in almost everyone. In a four year study, the Centers for Disease Control and Prevention found that the virus did not adhere to Koch's postulates and therefore had no definitive association between CFS and EBV but it is still being studied by researchers.

XMRV

In 2009, researchers published a paper in the journal Science announcing that in 67% of the samples of 101 chronic fatigue syndrome patients (as compared to 3.7% of 218 healthy controls), they had found a retrovirus called XMRV: xenotropic murine leukemia virus-related virus.17 However, numerous other labs trying to duplicate the results of the Science paper were unable to find the retrovirus in patients with the disease.18 In the wake of recent studies,19 editors for the journal Science have gone so far as to ask the original authors to retract their paper.20

For many scientists, the inability of other labs to confirm the results raised questions about the validity of the retroviral finding. Others such as W. Ian Lipkin have suggested that other factors may be to blame: varying definitions of CFS could contribute to conflicting reports, as well as differences in the way labs process blood samples, and which tests they use.

Enterobacteria and enteroviruses

A number of studies have detected enterobacteria in the gut of patients with CFS. Maes et al. found that the prevalences in response to the presences and median values for serum IgA against the lipopolysaccharides of enterobacteria are significantly greater in patients with CFS.21 The presence of chronic disseminated enteroviral infection, as determined by stomach or muscle biopsy, has also been identified in a subset of patients with the syndrome. Acquisition of these pathogens as part of the successive infection process may well contribute to common CFS “comorbidities” such as irritable bowel syndrome or sensitivities to certain foods.

Chronic fatigue syndrome is associated with chronic enterovirus infection of the stomach.22

Mycoplasma

Nijs et al. found a high prevalence of Mycoplasma infections among European chronic fatigue syndrome patients.23 In this prospective study, the presence of Mycoplasma fermentans, M. penetrans, M. pneumoniae and M. hominis was assessed in the blood of 261 European CFS patients and 36 healthy volunteers using forensic polymerase chain reaction. One hundred and seventy-nine (68.6%) patients were infected by at least one species of Mycoplasma, compared to two out of 36 (5.6%) in the control sample. Multiple mycoplasmal infections were detected in 45 patients (17.2%). Compared to American CFS patients (M. pneumoniae > M. hominis > M. penetrans), a slightly different pattern of mycoplasmal infections was found in European CFS patients (M. hominis > M. pneumoniae, M. fermentansz & M. penetrans).

Other infections

Certain other infectious illnesses, such as Q fever and viral meningitis are associated with a greater risk of developing CFS, but many people have no evidence of viral infection, and there is no evidence of persistent infection.24

Incline Village and other outbreaks

There are several reports of CFS outbreaks, one prominent example of which is Incline Village.

[The] clusters of post-infectious chronic fatigue syndrome are very interesting. Studies by Andrew Lloyd and colleagues25 showed the one and only predictor of persistent illness after an acute infection was the severity of that initial infection. No psychiatric factors were shown to predict who remains ill.

Some very common infections seem to set off C.F.S. and lead to several theories about the disease. My favorite theory is that any infection that can activate the immune system in a profound way could allow a hidden latent virus or viruses to reactivate and perpetuate a chronic illness. Anything that suppresses the immune system, like some treatments for cancer, might do the same thing, causing profound fatigue in some “recovered” cancer patients years after chemotherapy.

Nancy Klimas, M.D., New York Times

Length of the MP

The length of time it takes an MP patient to return to a given level of functionality seems to vary somewhat by disease. Diseases like CFS and Lyme may take longer. This may be because patients with these diseases tend to be sicker than most.

I think that, in general, CFS and Lyme patients have had more long-term intervention from supplements and pharmaceuticals than many of the sarcies. Additionally, their disease is not as apparent as, for example, lupus or sarcoidosis, and this means that they generally are more ill before they get help. A sarc patient, for example, may die of pulmonary insufficiency or cardiac arrest before their overall systemic organ status degenerates to that of some of the CFS and Lyme patients.

I don't think there is any fundamental difference in the species, however. I myself suffered from debilitating fatigue for almost a decade, and had to work my life around my unusual sleep and rest patterns. It was seeing that fatigue in the diabetes patients (whom I was researching) that first made me realize the common threads through all the Th1 diseases.

I do think it is important that CFS and Lyme patients do face up to the extent of their illness, however. Think about this - it takes 2 years or more for the bacteria to be killed, at the fastest rate your body can kill them. It is absolutely amazing what that quantity of bacteria must have been doing while they were living in your tissues

Trevor Marshall, PhD

Cognitive, behavioral and psychosocial explanations

Sigmund Freud and Jean-Martin Charcot were born 150 years ago, but their ideas about the effect of the subconscious on disease continue to resonate in the scientific community.26 Freud and colleagues argued that unconscious mental processes such as sublimated rage could manifest as physical symptoms. However, with the advent of superior technology, one by one, many diseases once supposed to be caused by psychological stress have since been attributed to other factors including infections.

According to the Marshall Pathogenesis, chronic fatigue syndrome, multiple chemical sensitivity and other chronic inflammatory diseases are likely caused by pathogens, yet many physicians consider these diseases to be “medically unexplained.” Medically unexplained diseases are widely prevalent27 but at the same time have few discernible markers or objectively measurable symptoms. While a lot of Freudian ideas have fallen out of favor, one legacy remains: difficult-to-explain diseases are still routinely attributed to psychological causes. The process by which patients supposedly manifest psychological problems as a disease has been named and renamed, classified and reclassified: hysteria, psychosomatic disorder, somatoform disorder, conversion disorder, functional disorder, etc. In each of these diagnoses, however, the stated origin of disease is unchanged: symptoms that cannot be explained are ultimately “all in a patient's head.”

While there is no denying the existence of some sort of “mind-body connection,” there is minimal compelling evidence that as the 19th century Swiss physician Georg W. Groddeck claimed: “Illness has a purpose; it has to resolve the conflict, to repress it, or to prevent what is already repressed from entering consciousness.”28 Despite the stark absence of evidence supporting these views, it is not unusual to read papers describing how patients with long-term so-called psychological illnesses may be subconsciously manifesting them, because it would allow them to have more “care, attention, disengagement, or even financial benefits.”29 Nor, is it uncommon for new theories to spring up along these lines. In one example, a 2008 continuing medical education publication taught physicians that when a celebrity becomes ill, healthy people are suggestible enough to develop long-term illnesses consistent with the celebrity's descriptions of their conditions. Such claims are recklessly speculative, harming patients and stalling needed research.

Treating patients who complain of so-called medically unexplained symptoms with cognitive behavioral therapy or, in the case of chronic fatigue syndrome, graded exercise therapy, may do more harm than good.30 The emergence of metagenomic technologies offers a more sophisticated set of tools for detecting and characterizing microbes in these disease states. Perhaps it is only the use of this technology that will finally relegate the notion of patient as attention-seeking victim to historical relic.

Behavioral explanations

CFS/ME patients' behaviors are routinely blamed for causing, if not driving, their illnesses, because they:

  • unnecessarily seek medical care – One study found CFS patients consulted their physician more frequently in the 15 years before development of their condition.31 This study proves only that CFS patients had signs of sickness (and noticed them) prior to diagnosis.
  • participate in social networks – A lack of support or the reinforcement of “illness behaviour” by social networks has been proposed to delay recovery for some patients.32
  • display “sickness behavior” – This demeaning turn of phrase was used in a 2010 study to suggest that patients with CFS/ME are acting as if they were sick.33 Sickness behavior is said to be reinforced by people in the patient's environment, such as a partner or family.34 35
  • display high levels of “action-proneness” – Van Houdenhove et al. employ this euphemism for saying patients were “hyperactive” prior to disease onset.36 37 This is analogous to claiming patients with obstructive lung diseases contract those illnesses by excessive breathing. According to this logic, unlike nearly every other disease, patients' dissatisfaction with being sick is responsible for them remaining sick.
  • engage in catastrophizing – A euphemism for fearing the worst.38
  • engage in avoidance behaviour – A pervasive pattern of social inhibition.39 Interestingly, this was used in a paper describing how CFS/ME patients have high levels of nitric oxide, a metabolite which plays a well-documented role in host surveillance.40 41

Cognitive explanations

According to a 2006 Lancet paper, “Inactivity of patients with CFS is caused by perceptions and expectations rather than by physical fitness.”42 Patients with the condition supposedly:

  • perceive symptoms inaccurately – Prins summed up the literature when she said there are discrepancies between perceived and actual cognitive performance, perceived and actual sleep disturbance, and perceived and actual functioning.43
  • fixate on a physical cause for symptoms or when exercise is avoided.44 45
  • are neurotic and introverted46 – However, a more recent systematic review of personality and CFS found an association with neuroticism, but stated that it was often accounted for by co-morbid depression and levels were similar to those in patients with other chronic diseases, and found no firm evidence for introversion.47
  • fail to self-regulate – Deary has hypothesized that in CFS/ME, the health threat is no longer the illness, but anything that threatens to disrupt a precarious accommodation to it.48 Due to established vicious circles, attempts at threat regulation may become inadvertently self-defeating, promoting the threats they attempt to diminish. By logical extension, CFS/ME patients who dismissed this argument would do so because they are sick and in so doing, continue to be sick. Or, in other words, failure to agree with a researcher's belief about an illness causes that illness.
  • engage in somatization – So called “somatoform diseases,” are conditions in the which internal psychological conflicts are unconsciously expressed as physical signs has also been implicated.49

Therapy based on the cognitive-behavioral model of disease

Cognitive behavioral therapy (CBT), graded exercise therapy (GET) and other interventions for CFS/ME are predicated on the profoundly flawed cognitive-behavioral model for the disease. They also suffer from bias. For example, because many of the CBT and GET studies required patients to visit a clinic, severely affected patients may have been excluded.50

  • cognitive behavioral therapy – CBT is a form of psychological therapy in which patients are taught to solve problems concerning dysfunctional emotions, behaviors and cognitions through a goal-oriented, systematic procedure. Being chronically ill can be very challenging, and professional counseling for diseases that have profound neurological components, is usually useful. But the whole point of this therapy is to “help” the patient develop behaviors and insights into why they are ultimately responsible for their illnesses.
  • graded exercise therapy – GET is a form of physical therapy in which patients being exercising very slowly and gradually increase exercise duration and intensity over time.

Because patients suffering from CFS/ME suffer from an organic cause, these interventions are unsettling, if not sadistic. It is tragic or humorous to think either therapy would ever be considered a first-line therapy for illnesses such as AIDS, leprosy or other diseases for which the infectious causes of which are less disputed.

We conclude that it is unethical to treat patients with ME/CFS with ineffective, non-evidence-based and potentially harmful “rehabilitation therapies”, such as CBT/GET.

F.N. Twisk, et al.51

As of November 2010, the top three treatments for CFS on the CDC website remain, respectively: professional counseling, cognitive behavioral therapy (CBT), and graded exercise therapy (GET).

Other treatments

  • Rituximab – In 2011, a Norwegian study published in PLoS One studied the effect of Rituximab – a monoclonal antibody otherwise used in cancers and anti-rejection treatment for organ transplants – on patients with CFS. In the 25-week study period, fatigue improved significantly in 10 out of 15 patients versus 2 out of 15 controls.52 This positive result may be due to the fact that Rituximab is a strong immunosuppressant. It may be telling to see a follow up of the patients who received the drug ten or even fifteen years from now.

Patient interviews

Peter de Jager

chronic fatigue syndrome (CFS), multiple chemical sensitivity

Read the interview

Paul Albert

chronic fatigue syndrome (CFS), depression, food sensitivities

Read the interview

Doreen V. (patient's mother)

autism, ADHD, depression, severe anxiety, chronic fatigue syndrome (CFS)

Read the interview

Carole Morgan

sarcoidosis, fibromyalgia, chronic fatigue syndrome (CFS)

Read the interview


Interviews of patients with other diseases are also available.

Read more

Keywords:

Notes and comments

Alternative explanations

cardiac insufficiency

  • sensitization – the process by which an organism is repeatedly exposed to a stimulus, progressively amplifying the response. Nijs et al. argue unconvincingly that elevated levels of nitric oxide (which coincidentally play a key role in host defense) sensitize the central nervous system to stimuli.53

XMRV infects human cells in culture, but not in-vivo

A new paper confirms my concerns about the Whittemore Peterson Institute using cell-cultures as part of their XMRV assay:

http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0015632

Note also that we have explained how important it is to select the correct primers for a nested-PCR analysis, something that many research groups do not yet seem to fully appreciate. It is on page 20 of our recent book chapter:

http://autoimmunityresearch.org/preprints/Proal_MHB_Chapter_preprint.pdf

..Trevor..

FYI,Interesting article which is receiving flak from the online CFS communities.

http://www.chicagotribune.com/health/ct-met-chronic-fatigue-xmrv-20110317,0,1822523,full.story

..Trevor..

Indeed, it would indeed be interesting to get a dump of the raw data, all of it, so we could look through it for associations other than those related to diagnoses, which in any case represent subsets of the syndromes underlying the systemic disease processes. I have read the full-text, and their Venn-diagram clearly shows a likelihood of more overlap if groups based on the diagnoses were not so slavishly followed. I would like to create a comparison of “sick vs healthy” for example. And you are correct, this fits exactly into the “imponderably complex” description of chronic disease processes which has resulted from our understanding of the metagenomic cause of this systemic dysfunction.

=========================================================================== Prof. Marshall's Members' Only Topics > XMRV retrovirus 'might be culprit' behind CFS ===========================================================================

From: Dr Trevor Marshall Date: 2010-12-20 10:44:36 Reply: http://www.marshallprotocol.com/reply.php?topic_id=13461

Interesting. Five new papers, published at once, confirm the hypothesis I advanced above. Namely that the cell cultures used for 'amplification' were contaminated. Interesting that the test kits themselves were apparently also contaminated.

Finally, one of the papers says that WPI may have made the mistake we warn about on page 257 of our Metagnenomics book chapter: “Primers selected for most epidemiological studies are chosen without consideration for whether they might amplify DNA from the genomes of any intracellular microbes. As artist Pablo Picasso once remarked, “Computers are useless. They can only give you answers.” If a software program fails to make provision for the possibility that a metagenome might also be present, the chances of a false positive increase significantly during the process of genomic analysis. Similarities between bacterial and human genes will likely cause the analysis software to not assemble the genomic data properly” A summary of the new articles can be found at:

http://www.guardian.co.uk/society/2010/dec/20/chronic-fatigue-me-research

..Trevor..

From: Dr Trevor MarshallDate: 2011-01-03 21:13:26 Reply: http://www.marshallprotocol.com/reply.php?topic_id=13461

Why wouldn't the samples from the controls be contaminated We are dealing with thousands of microbes making up the microbiome, of which perhaps hundreds are key to the dysfunctions in CFS. Microbes interact with other microbes, producing a thick genetic mix. The PCR testing which was used by WPH to identify the small strands of DNA/RNA which they believe to be representative of XMRV were not in fact very rigorous, an issue which is highlighted in this latest paper just published.

Perhaps the healthy immune system of controls could get rid of the murine-based viruses, and the weakened CFS immune system could not, leaving them to be found by WPH. There is real significance in the report that culture of the murine viruses was possible in-vitro, in a cell line, but not in-vivo, where the immune system was hard at work.

Sadly, I don't have first-hand knowledge of exactly how WPI conducted their study. I doubt anybody really does. I do believe that it was flawed and biased in at least one respect – epitomized by the blogger who wrote that Dr Peterson selected him for the trial cohort because he had an EBV infection. Perhaps the controls didn't have EBV? There are so many areas where a study can take a wrong turn and end up at the wrong point. Without going over every step it is not possible for me to second-guess every one of those turns :)

BMC Microbiol. 2011 Jan 2;11:2. An unbiased metagenomic search for infectious agents using monozygotic twins discordant for chronic fatigue. Sullivan PF, Allander T, Lysholm F, Goh S, Persson B, Jacks A, Evengård B, Pedersen NL, Andersson B. Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. pfsulliv@med.unc.edu Abstract BACKGROUND: Chronic fatigue syndrome is an idiopathic syndrome widely suspected of having an infectious or immune etiology. We applied an unbiased metagenomic approach to try to identify known or novel infectious agents in the serum of 45 cases with chronic fatigue syndrome or idiopathic chronic fatigue. Controls were the unaffected monozygotic co-twins of cases, and serum samples were obtained at the same place and time. RESULTS: No novel DNA or RNA viral signatures were confidently identified. Four affected twins and no unaffected twins evidenced viremia with GB virus C (8.9% vs. 0%, p = 0.019), and one affected twin had previously undetected hepatitis C viremia. An excess of GB virus C viremia in cases with chronic fatigue requires confirmation. CONCLUSIONS: Current, impairing chronic fatigue was not robustly associated with viremia detectable in serum. PMID: 21194495

On page 5: “background contamination (mostly human or bacterial)”(my emphasis)

Sanger sequencing is least suitable for metagenomic analysis, as it requires short reads. Additionally, they didn't perform enough reads, IMO.

Interesting paper, though… it would imply that a virus is probably not the primary cause of CFS…

..Trevor..

Med Hypotheses. 2011 Apr 5. [Epub ahead of print]

Chronic fatigue syndrome -A neuroimmunological model.

Arnett SV, Alleva LM, Korossy-Horwood R, Clark IA.

Research School of Biology, Australian National University, Australia.

Abstract

The aetiological and pathophysiological basis of chronic fatigue syndrome (CFS) remains a controversial field of inquiry in the research community.

While CFS and similar disease conditions such as fibromyalgia (FM) and post-infectious encephalopathy have been the focus of intense scrutiny for the past 20 years, results of research were often contradictory and a cohesive pathological model has remained elusive.

However, recent developments in understanding the unique immunophysiology of the brain may provide important clues for the development of a truly comprehensive explanation of the pathology of CFS.

We argue that CFS pathogenesis lies in the influence of peripheral inflammatory events on the brain and the unique immunophysiology of the central nervous system.

There is also evidence that CFS patients have a relative immunodeficiency that predisposes to poor early control of infection that leads to chronic inflammatory responses to infectious insults.

The neurological and endocrine changes have been described in CFS patients support the view that CFS has an inflammatory pathogenesis when considered as a whole.

An inflammatory model of disease also provides an explanation for the marked female sex bias associated with CFS.

This review therefore posits the hypothesis that CFS as a disease of long-term inflammatory processes of the brain.

We will also provide an investigative framework that could be used to justify the use of anti-TNF biological agents as a reliable and effective treatment approach to CFS, a syndrome that to date remains frustratingly difficult for both patients and health care professionals to manage.

Copyright © 2011 Elsevier Ltd. All rights reserved.

PMID: 21474251 [PubMed - as supplied by publisher]

From: Bane Date: 2011-05-04 15:41:39 Reply: http://www.marshallprotocol.com/reply.php?topic_id=13461

Chronic Fatigue Syndrome Is Not Related to XMRV Retrovirus, Comprehensive Study Finds

http://healthcare.utah.edu/publicaffairs/news/current/05411Singh.html

“Our investigation found no trace of XMRV in any of the blood samples taken from patients we obtained ourselves, or from patients previously tested in the 2009 Science study,” Singh said.  “Because of our findings, we believe chronic fatigue syndrome patients should reconsider the merit of taking antiretroviral agents to alleviate their symptoms.”

From: Phillyguy Date: 2011-05-04 20:51:51 Reply: http://www.marshallprotocol.com/reply.php?topic_id=13461

The patients on the CFS boards are very angry about the interpretation of these results.  However, what they dont realize is that this is good news.  Retroviruses can be really, really nasty and difficult to treat.

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