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home:pathogenesis:vitamind:metabolism [01.01.2019] – [Metabolism of vitamin D and the Vitamin D Receptor] sallieqhome:pathogenesis:vitamind:metabolism [09.14.2022] (current) – external edit 127.0.0.1
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 Located in the nucleus of a variety of cells including immune cells, the VDR is a control system of sorts. When exposed to infection and damage, especially that which is caused by pathogens, the body begins to convert the inactive form 25-D into the active form, 1,25-D. As cellular concentrations of 1,25-D increase, 1,25-D activates the VDR, turning on any number of genes the receptor transcribes.  Located in the nucleus of a variety of cells including immune cells, the VDR is a control system of sorts. When exposed to infection and damage, especially that which is caused by pathogens, the body begins to convert the inactive form 25-D into the active form, 1,25-D. As cellular concentrations of 1,25-D increase, 1,25-D activates the VDR, turning on any number of genes the receptor transcribes. 
- [[https://autoimmunityresearch.org/hormones.pdf|Hormonal changes result from change in 1,25 dihydroxyvitamin-D]]+  
 +[[https://autoimmunityresearch.org/hormones.pdf|Hormonal changes result from change in 1,25 dihydroxyvitamin-D]]
  
-According to a 2010 analysis, the VDR significantly affects 229 human genes. Many of these genes have long been associated with autoimmune diseases and cancers including, for example, the genes IRF8 (linked to multiple sclerosis), and PTPN2 (connected to Crohn's disease and type I diabetes).(({{pubmed>long:20736230}})) The activation of certain genes also leads to the synthesis of antimicrobial peptides. The antimicrobial peptides are the body's "natural antibiotics" and have a potent anti-bacterial effect.+According to a 2010 analysis, the VDR significantly affects 229 human genes. Many of these genes have long been associated with autoimmune diseases and cancers including, for example, the genes IRF8 (linked to multiple sclerosis), and PTPN2 (connected to Crohn's disease and type I diabetes).(({{pmid>long:20736230}})) The activation of certain genes also leads to the synthesis of antimicrobial peptides. The antimicrobial peptides are the body's "natural antibiotics" and have a potent anti-bacterial effect.
  
 However, bacteria create ligands, which like 25-D, inactivate the VDR and, in turn, the innate immune response. This allows the microbes to proliferate. In response, the body increases production of 1,25-D from 25-D, leading to one of the hallmarks of chronic inflammatory disease: a low 25-D and a high 1,25-D.  However, bacteria create ligands, which like 25-D, inactivate the VDR and, in turn, the innate immune response. This allows the microbes to proliferate. In response, the body increases production of 1,25-D from 25-D, leading to one of the hallmarks of chronic inflammatory disease: a low 25-D and a high 1,25-D. 
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 [{{ :home:pathogenesis:vitamind:25dvs125d.gif|**1,25-D is different than 25-D in that it possesses a single 1-alpha hydroxylation.** It is this additional hydroxylation, which stabilizes helix 12 in the Vitamin D Receptor binding the promoter which allow activation of the VDR and leads to the transcription of thousands of genes.}}] [{{ :home:pathogenesis:vitamind:25dvs125d.gif|**1,25-D is different than 25-D in that it possesses a single 1-alpha hydroxylation.** It is this additional hydroxylation, which stabilizes helix 12 in the Vitamin D Receptor binding the promoter which allow activation of the VDR and leads to the transcription of thousands of genes.}}]
  
-A general appreciation for how 25-D and 1,25-D compete for nuclear receptors gets to the heart of their opposing roles in the body. According to the Marshall Pathogenesis, the VDR is foremost a control system. Under most circumstances, the active form, 1,25-D, acts as the "on" switch and the inactive form, 25-D, is the "off" switch.(({{pubmed>long:18200565}})) 25-D is not completely inactive, but it does not and cannot activate the VDR. As Leow states in //Respirology//, "25-D levels are not associated with levels of cathelicidin or beta-defensin-2 [antimicrobial proteins transcribed by the VDR]."(({{pubmed>long:21244571}}))+A general appreciation for how 25-D and 1,25-D compete for nuclear receptors gets to the heart of their opposing roles in the body. According to the Marshall Pathogenesis, the VDR is foremost a control system. Under most circumstances, the active form, 1,25-D, acts as the "on" switch and the inactive form, 25-D, is the "off" switch.(({{pmid>long:18200565}})) 25-D is not completely inactive, but it does not and cannot activate the VDR. As Leow states in //Respirology//, "25-D levels are not associated with levels of cathelicidin or beta-defensin-2 [antimicrobial proteins transcribed by the VDR]."(({{pmid>long:21244571}}))
  
-Further underscoring this role for these two D metabolites is that 25-D and 1,25-D "happen" to share similar binding affinities for the VDR. According to molecular modeling by Trevor Marshall, PhD, 1,25-D has an affinity of 8.48 (as measured by nanomolar Kd) and 25-D has an affinity of 8.36.(({{pubmed>long:18200565}})) It would seem that activation of the Vitamin D nuclear receptor is achieved by a delicate balance between the concentrations of a number of endogenous hormones. Indeed, at the risk of overgeneralization, the body increases and decreases the production of 1,25-D to control the innate immune response.+Further underscoring this role for these two D metabolites is that 25-D and 1,25-D "happen" to share similar binding affinities for the VDR. According to molecular modeling by Trevor Marshall, PhD, 1,25-D has an affinity of 8.48 (as measured by nanomolar Kd) and 25-D has an affinity of 8.36.(({{pmid>long:18200565}})) It would seem that activation of the Vitamin D nuclear receptor is achieved by a delicate balance between the concentrations of a number of endogenous hormones. Indeed, at the risk of overgeneralization, the body increases and decreases the production of 1,25-D to control the innate immune response.
  
 As mentioned before, exposure to injury and infection enhances production of 1,25-D, which in turn leads to the creation of antimicrobial peptides and activation of TLR2. As mentioned before, exposure to injury and infection enhances production of 1,25-D, which in turn leads to the creation of antimicrobial peptides and activation of TLR2.
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 However, certain feedback mechanisms are also in place, which allow the body to limit the production of 1,25-D to just that amount needed for proper transcriptional activation of the VDR. However, certain feedback mechanisms are also in place, which allow the body to limit the production of 1,25-D to just that amount needed for proper transcriptional activation of the VDR.
   * When the VDR is activated, it transcribes the gene for the enzyme CYP24A1, which increases conversion of 1,25-D into inactive metabolites.   * When the VDR is activated, it transcribes the gene for the enzyme CYP24A1, which increases conversion of 1,25-D into inactive metabolites.
-  * An activated VDR also controls 1,25-D concentration by limiting transcription of the gene CYP27B1, which converts 25-D into 1,25-D.(({{pubmed>long:17368181}})) +  * An activated VDR also controls 1,25-D concentration by limiting transcription of the gene CYP27B1, which converts 25-D into 1,25-D.(({{pmid>long:17368181}})) 
  
 ===== Bacteria and the VDR ===== ===== Bacteria and the VDR =====
  
-The [[http://aps.unmc.edu/AP/main.php|Antimicrobial Peptide Database]] lists hundreds of antimicrobial peptides known to kill or inhibit the reproduction of bacteria,(({{pubmed>long:18957441}})) 793 AmPs found in animals as of January 5, 2009. The sheer diversity of these proteins coupled with the fact that they have been conserved over millenia suggests that enough pathogenic bacteria exist in sufficient quantities to warrant the evolution of these defense mechanisms. It would seem that is in the strong interest of the human body to destroy or disrupt these bacteria.+The [[https://aps.unmc.edu/AP/main.php|Antimicrobial Peptide Database]] lists hundreds of antimicrobial peptides known to kill or inhibit the reproduction of bacteria,(({{pmid>long:18957441}})) 793 AmPs found in animals as of January 5, 2009. The sheer diversity of these proteins coupled with the fact that they have been conserved over millenia suggests that enough pathogenic bacteria exist in sufficient quantities to warrant the evolution of these defense mechanisms. It would seem that is in the strong interest of the human body to destroy or disrupt these bacteria.
  
 Pathogenic bacteria are likewise driven by evolutionary impetus: it's in their interest to disrupt the proteins, which interfere with their growth.  In what way or ways could bacteria interrupt production of the AmPs? Pathogenic bacteria are likewise driven by evolutionary impetus: it's in their interest to disrupt the proteins, which interfere with their growth.  In what way or ways could bacteria interrupt production of the AmPs?
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 <blockquote>Acquisition of resistance by a sensitive microbial strain against antimicrobial peptides is surprisingly improbable. <blockquote>Acquisition of resistance by a sensitive microbial strain against antimicrobial peptides is surprisingly improbable.
  
-//**Michael Zasloff**// (({{pubmed>long:11807545}})) </blockquote>+//**Michael Zasloff**// (({{pmid>long:11807545}})) </blockquote>
    
 However, what if it were possible to disrupt the expression of the Vitamin D Receptor by secreting ligands, which bind to and inactivate the receptor? Such bacteria would have an undeniable reproductive advantage. However, what if it were possible to disrupt the expression of the Vitamin D Receptor by secreting ligands, which bind to and inactivate the receptor? Such bacteria would have an undeniable reproductive advantage.
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 [{{ :home:pathogenesis:vitamind:capninelarge.jpg?300|**Capnine is a protein created by bacteria, a protein which may bind to and antagonize (inactivate) the VDR.** The secretion of capnine and substances like it fulfills an important evolutionary need for bacteria: to disrupt the innate immune response.}}] [{{ :home:pathogenesis:vitamind:capninelarge.jpg?300|**Capnine is a protein created by bacteria, a protein which may bind to and antagonize (inactivate) the VDR.** The secretion of capnine and substances like it fulfills an important evolutionary need for bacteria: to disrupt the innate immune response.}}]
  
-In the arms race of host–microbe co-evolution, successful microbial pathogens have evolved ingenious ways to evade host immune responses.(({{pubmed>long:21350579}}))+In the arms race of host–microbe co-evolution, successful microbial pathogens have evolved ingenious ways to evade host immune responses.(({{pmid>long:21350579}}))
  
 <blockquote>Studies have indicated that the dysregulation of VDR may lead to exaggerated inflammatory responses, raising the possibility that defects in Vitamin D and VDR signaling transduction may be linked to bacterial infection and chronic inflammation. Further characterization of Vitamin D/VDR will help elucidate the pathogenesis of various human diseases and in the design of new approaches for prevention and treatment. <blockquote>Studies have indicated that the dysregulation of VDR may lead to exaggerated inflammatory responses, raising the possibility that defects in Vitamin D and VDR signaling transduction may be linked to bacterial infection and chronic inflammation. Further characterization of Vitamin D/VDR will help elucidate the pathogenesis of various human diseases and in the design of new approaches for prevention and treatment.
  
-//**Jun Sun**// (({{pubmed>long:20639756}}))</blockquote>+//**Jun Sun**// (({{pmid>long:20639756}}))</blockquote>
  
  
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 In keeping with evolutionary theory, a growing number of substances and species have been shown to downregulate the activity of the VDR: In keeping with evolutionary theory, a growing number of substances and species have been shown to downregulate the activity of the VDR:
-  * **//Borrelia burgdorferi//** – Live //Borrelia burgdorferi// reduced VDR expression in monocytes (phagocytes) by 50 times, and lysates ("dead" //Borrelia//) reduced it by 8 times(({{pubmed>long:19461888}}))    +  * **//Borrelia burgdorferi//** – Live //Borrelia burgdorferi// reduced VDR expression in monocytes (phagocytes) by 50 times, and lysates ("dead" //Borrelia//) reduced it by 8 times(({{pmid>long:19461888}}))    
-  * **//Mycobacterium tuberculosis//** – shown to downregulate the VDR 3.3-fold (({{pubmed>long:12890386}})) which makes sense given that an active VDR helps phagocytes to suppress the intracellular growth of //M. tuberculosis// (({{pubmed>long:11461902}})) (({{pubmed>long:9784538}})) +  * **//Mycobacterium tuberculosis//** – shown to downregulate the VDR 3.3-fold (({{pmid>long:12890386}})) which makes sense given that an active VDR helps phagocytes to suppress the intracellular growth of //M. tuberculosis// (({{pmid>long:11461902}})) (({{pmid>long:9784538}})) 
-  * Mycobacterium tuberculosis enzyme involved in vitamin D and 7-dehydrocholesterol metabolism (({{pubmed>long:27289046}})) +  * Mycobacterium tuberculosis enzyme involved in vitamin D and 7-dehydrocholesterol metabolism (({{pmid>long:27289046}})) 
-  * **//Mycobacterium leprase//** – produces microRNA-21 to target multiple genes associated with the VDR(({{pubmed>long:22286305}})) +  * **//Mycobacterium leprase//** – produces microRNA-21 to target multiple genes associated with the VDR(({{pmid>long:22286305}})) 
-  * **"Gliding" biofilm bacteria have been shown to create Capnine** – Capnine is a 2-amino-3-hydroxy-15-methylhexadecane-1-sulfonic acid, and is created by the genera //Cytophaga, Capnocytophaga, Sporocytophaga,// and //Flexibacter//.(({{pubmed>long:2992489}})) (({{pubmed>long:6330048}})) The secretion of capnine meets an important evolutionary need for bacteria. Capnine possesses a high affinity for the VDR as evidenced by the fact that molecular modeling shows that its stable in the ligand binding pocket. [[home:publications:marshall_metagenomics_2007|Molecular modeling]] further shows that when capnine is docked in the VDR, it inactivates the receptor.+  * **"Gliding" biofilm bacteria have been shown to create Capnine** – Capnine is a 2-amino-3-hydroxy-15-methylhexadecane-1-sulfonic acid, and is created by the genera //Cytophaga, Capnocytophaga, Sporocytophaga,// and //Flexibacter//.(({{pmid>long:2992489}})) (({{pmid>long:6330048}})) The secretion of capnine meets an important evolutionary need for bacteria. Capnine possesses a high affinity for the VDR as evidenced by the fact that molecular modeling shows that its stable in the ligand binding pocket. [[home:publications:marshall_metagenomics_2007|Molecular modeling]] further shows that when capnine is docked in the VDR, it inactivates the receptor.
   * **//Chlamydia trachomatis//** – shown to downregulate the VDR in unpublished work   * **//Chlamydia trachomatis//** – shown to downregulate the VDR in unpublished work
    
 The following substances reduce the number of VDR, without which immune function is limited: The following substances reduce the number of VDR, without which immune function is limited:
-  * **Caspase-3** – A protein which cleaves (breaks apart) the VDR structure and thus limits the ability of VDR to perform gene transcription.(({{pubmed>long:18832097}})) The caspases are upregulated by //Shigella// infection,(({{pubmed>long:17696608}})) which implies that the VDR may have a faster cycle time in disease than in health.+  * **Caspase-3** – A protein which cleaves (breaks apart) the VDR structure and thus limits the ability of VDR to perform gene transcription.(({{pmid>long:18832097}})) The caspases are upregulated by //Shigella// infection,(({{pmid>long:17696608}})) which implies that the VDR may have a faster cycle time in disease than in health.
      
 According to the Marshall Pathogenesis, pathogens' production of ligands, which bind to and antagonize (inactivate) the Vitamin D Receptor, is one of the fundamental processes by which chronic inflammatory disease occurs. The consumption of other immunosuppressive substances also has an effect. According to the Marshall Pathogenesis, pathogens' production of ligands, which bind to and antagonize (inactivate) the Vitamin D Receptor, is one of the fundamental processes by which chronic inflammatory disease occurs. The consumption of other immunosuppressive substances also has an effect.
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 One can see the effects of a dysfunctional VDR in knockout mice, mice genetically engineered to be born without the receptor. These mice demonstrate what it is like to have a VDR completely blocked by bacterial ligands. One can see the effects of a dysfunctional VDR in knockout mice, mice genetically engineered to be born without the receptor. These mice demonstrate what it is like to have a VDR completely blocked by bacterial ligands.
  
-Mice without a VDR have been shown in separate studies to be born with alopecia, an inflammatory condition in which organisms have no hair(({{pubmed>long:17517646}})) and age prematurely.(({{pubmed>long:19500727}})) Scientists have also found that //Salmonella// is much more virulent and aggressive in mice in which the vitamin D receptor had been turned off.(({{pubmed>long:20639756}})) These mice showed higher levels of activity of inflammatory molecules, and they lost weight more quickly and were much more likely to die in response to infection.+Mice without a VDR have been shown in separate studies to be born with alopecia, an inflammatory condition in which organisms have no hair(({{pmid>long:17517646}})) and age prematurely.(({{pmid>long:19500727}})) Scientists have also found that //Salmonella// is much more virulent and aggressive in mice in which the vitamin D receptor had been turned off.(({{pmid>long:20639756}})) These mice showed higher levels of activity of inflammatory molecules, and they lost weight more quickly and were much more likely to die in response to infection.
  
  
-Further validating the Marshall Pathogenesis model is this: other research in VDR knockout mice has shown a marked increase, by a factor of ten, in serum 1,25-D and a clear reduction - to almost undetectable levels - in serum 25-D. Such levels persisted at seven weeks until the mice eventually died.(({{pubmed>long:9241280}}))+Further validating the Marshall Pathogenesis model is this: other research in VDR knockout mice has shown a marked increase, by a factor of ten, in serum 1,25-D and a clear reduction - to almost undetectable levels - in serum 25-D. Such levels persisted at seven weeks until the mice eventually died.(({{pmid>long:9241280}}))
  
 ==== Mechanisms by which bacteria affect levels of 25-D and 1,25-D ==== ==== Mechanisms by which bacteria affect levels of 25-D and 1,25-D ====
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 ===== Viruses and fungi also affect the VDR ===== ===== Viruses and fungi also affect the VDR =====
  
-  * **Epstein-Barr virus (EBV)** – shown to downregulate expression of the VDR (and by the VDR) by a factor of about five, inducing "eventual immortalization"(({{pubmed>long:19550398}})) A 2011 paper further showed that a  EBV not only down-regulates expression of the VDR protein itself, but also acts to block transcription by the VDR.(({{pubmed>long:20593215}})) +  * **Epstein-Barr virus (EBV)** – shown to downregulate expression of the VDR (and by the VDR) by a factor of about five, inducing "eventual immortalization"(({{pmid>long:19550398}})) A 2011 paper further showed that a  EBV not only down-regulates expression of the VDR protein itself, but also acts to block transcription by the VDR.(({{pmid>long:20593215}})) 
-  * **HIV** – binds to the VDR(({{pubmed>long:9814454}})) and inhibits conversion of 25-D into 1,25-D(({{pubmed>long:19209727}})) (see below)  +  * **HIV** – binds to the VDR(({{pmid>long:9814454}})) and inhibits conversion of 25-D into 1,25-D(({{pmid>long:19209727}})) (see below)  
-  * **//Aspergillus fumigatus//** – In cystic fibrosis patients, the fungus //A. fumigatus// has been shown to secrete gliotoxin, a toxin which dose-dependently downregulates VDR mRNA and protein levels. This directly results in decreased levels of the AmP LL-37, thereby "providing an opportunistic environment for both bacterial and fungal colonization."(({{pubmed>long:22904183}})) ((Coughlan C.A. //et al.// [[http://ajrccm.atsjournals.org/cgi/reprint/181/1_MeetingAbstracts/A6184|Downregulation Of The 1α, 25 Dihydroxyvitamin-D3 (Vitamin D3) Receptor By Aspergillus Fumigatus Secreted Gliotoxin In Cystic Fibrosis]] //Am J Respir Crit Care Med// 181;2010:A6184.)) +  * **//Aspergillus fumigatus//** – In cystic fibrosis patients, the fungus //A. fumigatus// has been shown to secrete gliotoxin, a toxin which dose-dependently downregulates VDR mRNA and protein levels. This directly results in decreased levels of the AmP LL-37, thereby "providing an opportunistic environment for both bacterial and fungal colonization."(({{pmid>long:22904183}})) ((Coughlan C.A. //et al.// [[https://ajrccm.atsjournals.org/cgi/reprint/181/1_MeetingAbstracts/A6184|Downregulation Of The 1α, 25 Dihydroxyvitamin-D3 (Vitamin D3) Receptor By Aspergillus Fumigatus Secreted Gliotoxin In Cystic Fibrosis]] //Am J Respir Crit Care Med// 181;2010:A6184.)) 
-  * **Cytomegalovirus** – Cytomegalovirus affects hundreds of genes including downregulating the VDR 2.2 fold.(({{pubmed>long:18566437}})) +  * **Cytomegalovirus** – Cytomegalovirus affects hundreds of genes including downregulating the VDR 2.2 fold.(({{pmid>long:18566437}})) 
-  * **Hepatitis C virus** – Gal-Tanamy //et al.// showed that HCV infections interfere with the VDR by inhibiting the creation of CYP24A1, the enzyme responsible for breaking down excess 1,25-D.(({{pubmed>long:21793032}}))+  * **Hepatitis C virus** – Gal-Tanamy //et al.// showed that HCV infections interfere with the VDR by inhibiting the creation of CYP24A1, the enzyme responsible for breaking down excess 1,25-D.(({{pmid>long:21793032}}))
    
 ===== Evidence for high 1,25-D in patients with chronic disease ===== ===== Evidence for high 1,25-D in patients with chronic disease =====
  
-Under most normal conditions,  serum levels of 1,25-dihydroxyvitamin D are constant throughout the year (no variability due to sun exposure), but there is no such tight biochemical regulation in at least some chronic inflammatory diseases such as obesity.(({{pubmed>long:19444938}})) +Under most normal conditions,  serum levels of 1,25-dihydroxyvitamin D are constant throughout the year (no variability due to sun exposure), but there is no such tight biochemical regulation in at least some chronic inflammatory diseases such as obesity.(({{pmid>long:19444938}})) 
  
  
 ==== At the site of infection ==== ==== At the site of infection ====
  
-It is sometimes thought that the liver and kidney are the only sites for conversion of 25-D into 1,25-D, but there is evidence that this process happens outside those organs – not coincidentally, at the very sites where patients report symptoms of chronic disease. High levels of 1,25-D and the enzyme which leads to the production of 1,25-D, 1 α-hydroxylase, have been found at various locations where the human body needs a strong host defense.(({{pubmed>long:18981129}}))+It is sometimes thought that the liver and kidney are the only sites for conversion of 25-D into 1,25-D, but there is evidence that this process happens outside those organs – not coincidentally, at the very sites where patients report symptoms of chronic disease. High levels of 1,25-D and the enzyme which leads to the production of 1,25-D, 1 α-hydroxylase, have been found at various locations where the human body needs a strong host defense.(({{pmid>long:18981129}}))
  
-  * **skin cells of sarcoidosis patients** – Sarcoidosis patients have a variety of skin symptoms including bumps, ulcers, or discolored skin. Zehnder //et al// found increased expression of the enzyme 1 α-hydroxylase – the enzyme which converts 25-D into 1,25-D – in the skin cells of sarcoidosis patients.(({{pubmed>long:11158062}})) They write: +  * **skin cells of sarcoidosis patients** – Sarcoidosis patients have a variety of skin symptoms including bumps, ulcers, or discolored skin. Zehnder //et al// found increased expression of the enzyme 1 α-hydroxylase – the enzyme which converts 25-D into 1,25-D – in the skin cells of sarcoidosis patients.(({{pmid>long:11158062}})) They write: 
  
  <blockquote> In particular, the expression of 1α-OHase [1 α-hydroxylase] by activated macrophages and epidermal keratinocytes [skin cells] suggests a role for 1,25(OH)2D3 [1,25-D] as an immunomodulatory and/or antiproliferative hormone. </blockquote>   <blockquote> In particular, the expression of 1α-OHase [1 α-hydroxylase] by activated macrophages and epidermal keratinocytes [skin cells] suggests a role for 1,25(OH)2D3 [1,25-D] as an immunomodulatory and/or antiproliferative hormone. </blockquote> 
  
-  * **synovial fluid surrounding the joints of patients with rheumatoid arthritis** – Mawer //et al// found that 1,25-D levels were particularly elevated in the synovial fluid surrounding the joints of patients with rheumatoid arthritis (RA).(({{pubmed>long:1950677}})) In this study, median serum levels of 1,25-D at baseline was not elevated in the RA patients — only 24 pg/ml. Thus, the extrarenal synthesis of 1,25-D was not obvious from the routine blood test for 1,25-D. There is no reason to think that the metabolism of other diseases is any different.  +  * **synovial fluid surrounding the joints of patients with rheumatoid arthritis** – Mawer //et al// found that 1,25-D levels were particularly elevated in the synovial fluid surrounding the joints of patients with rheumatoid arthritis (RA).(({{pmid>long:1950677}})) In this study, median serum levels of 1,25-D at baseline was not elevated in the RA patients — only 24 pg/ml. Thus, the extrarenal synthesis of 1,25-D was not obvious from the routine blood test for 1,25-D. There is no reason to think that the metabolism of other diseases is any different.  
-  * **immune cells including macrophages** – Research has also shown that 1,25-D is synthesized in cells of the immune system, including the T cells and antigen-presenting cells(({{pubmed>long:14696037}})) as well as the macrophages.(({{pubmed>long:11158062}})) (({{pubmed>long:12887108}})) The fact that the immune cells are a site for 1,25-D synthesis is notable, because it is these cell types, especially macrophages, which are often infected by the Th1 pathogens. +  * **immune cells including macrophages** – Research has also shown that 1,25-D is synthesized in cells of the immune system, including the T cells and antigen-presenting cells(({{pmid>long:14696037}})) as well as the macrophages.(({{pmid>long:11158062}})) (({{pmid>long:12887108}})) The fact that the immune cells are a site for 1,25-D synthesis is notable, because it is these cell types, especially macrophages, which are often infected by the Th1 pathogens. 
  
  <blockquote> There is strong evidence that the extrarenal enzyme located in macrophages plays a major role in certain granulomatous conditions (e.g., sarcoidosis), causing uncontrolled elevations of blood 1,25-(OH)2D3 levels....   <blockquote> There is strong evidence that the extrarenal enzyme located in macrophages plays a major role in certain granulomatous conditions (e.g., sarcoidosis), causing uncontrolled elevations of blood 1,25-(OH)2D3 levels.... 
  
-//**Glenville** et al.//(({{pubmed>long:9790574}})) </blockquote> +//**Glenville** et al.//(({{pmid>long:9790574}})) </blockquote> 
  
-  * **respiratory epithelial cells** – The primary lung epithelial cells, which play an important role in the defense against airborne pathogens, have been shown to express high baseline levels of activating 1 α-hydroxylase and low levels of inactivating 24-hydroxylase. This activity leads to increased expression of genes by the Vitamin D Receptor.(({{pubmed>long:18981129}}))+  * **respiratory epithelial cells** – The primary lung epithelial cells, which play an important role in the defense against airborne pathogens, have been shown to express high baseline levels of activating 1 α-hydroxylase and low levels of inactivating 24-hydroxylase. This activity leads to increased expression of genes by the Vitamin D Receptor.(({{pmid>long:18981129}}))
  
  
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 A number of studies have demonstrated that the level of the hormone 1,25-D rises in patients with certain chronic diseases.  A number of studies have demonstrated that the level of the hormone 1,25-D rises in patients with certain chronic diseases. 
  
-  * **autoimmune disease** – Greg Blaney MD, a physician who practices in Vancouver, British Columbia (a setting with relatively infrequent sunlight), found that of a group of 100 patients with a variety of chronic inflammatory diseases, 85 had elevated measures of 1,25-D, which was defined as greater than or equal to 110 pmol/L.(({{pubmed>long:19758177}})) +  * **autoimmune disease** – Greg Blaney MD, a physician who practices in Vancouver, British Columbia (a setting with relatively infrequent sunlight), found that of a group of 100 patients with a variety of chronic inflammatory diseases, 85 had elevated measures of 1,25-D, which was defined as greater than or equal to 110 pmol/L.(({{pmid>long:19758177}})) 
-  * **Crohn's disease** – One study found that in a cohort of 88 Crohn's disease patients, 35 patients or 40% had elevated levels of 1,25-D, which the authors defined as above 60 pg/mL.(({{pubmed>long:15247180}})) +  * **Crohn's disease** – One study found that in a cohort of 88 Crohn's disease patients, 35 patients or 40% had elevated levels of 1,25-D, which the authors defined as above 60 pg/mL.(({{pmid>long:15247180}})) 
-  * **obesity** – Moan //et al.// showed that, in contrast to healthy people, patients with a high body mass index (BMI) had a significant seasonal variation in, not only  25(OH) vitamin D, but also of 1,25-D.(({{pubmed>long:19444938}}))  +  * **obesity** – Moan //et al.// showed that, in contrast to healthy people, patients with a high body mass index (BMI) had a significant seasonal variation in, not only  25(OH) vitamin D, but also of 1,25-D.(({{pmid>long:19444938}}))  
-  * **sarcoidosis** – Kavathia //et al// found that in patients with sarcoidosis, those with high serum levels of 1,25-D have more pronounced chronic treatment needs.(({{pubmed>long:20071158}})) +  * **sarcoidosis** – Kavathia //et al// found that in patients with sarcoidosis, those with high serum levels of 1,25-D have more pronounced chronic treatment needs.(({{pmid>long:20071158}})) 
-  * **asthma** – Liu //et al.// showed that levels of vitamin D metabolites, particularly 1,25-D, were low within the airways and increased after allergen challenge. The increases correlated with the magnitude of inflammation and increases in cathelicidin.(({{pubmed>long:22092530}}))+  * **asthma** – Liu //et al.// showed that levels of vitamin D metabolites, particularly 1,25-D, were low within the airways and increased after allergen challenge. The increases correlated with the magnitude of inflammation and increases in cathelicidin.(({{pmid>long:22092530}}))
  
-Bell listed the following diseases and conditions, which manifest with high levels of 1,25-D:  tuberculosis, AIDS with Pneumocystis carinii pneumonia, AIDS with cytomegalovirus infection, disseminated candidiasis, leprosy, rheumatoid arthritis, silicone-induced granulomas, Wegerner’s granulomatosis, Hodgkin’s disease, lymphoma, histocytic lymphoma, T-cell leukemia, plasma cell granuloma, leiomyoblastoma, seminoma, and subcutaneous fat necrosis.(({{pubmed>long:9525334}}))+Bell listed the following diseases and conditions, which manifest with high levels of 1,25-D:  tuberculosis, AIDS with Pneumocystis carinii pneumonia, AIDS with cytomegalovirus infection, disseminated candidiasis, leprosy, rheumatoid arthritis, silicone-induced granulomas, Wegerner’s granulomatosis, Hodgkin’s disease, lymphoma, histocytic lymphoma, T-cell leukemia, plasma cell granuloma, leiomyoblastoma, seminoma, and subcutaneous fat necrosis.(({{pmid>long:9525334}}))
 ===== Effect of high 1,25-D on nuclear receptors ===== ===== Effect of high 1,25-D on nuclear receptors =====
  
 <blockquote>In our study, the response to 1,25(OH)2D3 appears to be biphasic with a stimulatory effect at lower concentrations, and becoming inhibitory or ineffective at the higher levels. <blockquote>In our study, the response to 1,25(OH)2D3 appears to be biphasic with a stimulatory effect at lower concentrations, and becoming inhibitory or ineffective at the higher levels.
  
-//**Saveria Aquila** et al.//(({{pubmed>long:19948036}}))+//**Saveria Aquila** et al.//(({{pmid>long:19948036}}))
 </blockquote> </blockquote>
  
-At normal levels, the active vitamin D metabolite, 1,25-D, serves an important role in host defense,(({{pubmed>long:19943126}})) but high levels of the hormone are immunosuppressive(({{pubmed>long:19758324}}))  – if for no other reason than the fact that it is calcitriol (1,25-D) and its analogues are used widely to treat autoimmune disease. One of the mechanisms by which 1,25-D may be immunosuppressive (and contribute to symptoms of disease) is by interacting with the body's other nuclear receptors. Selvaraj //et al.// have suggested that the high levels of 1,25-D seen in patients with pulmonary tuberculosis "might lead to downregulation of VDR expression" and that "decreased VDR levels could result in defective VDR signaling."(({{pubmed>long:19219539}})) More recently, Johan Lundqvist's 2011 study showed that, consistent with the 2009 study by Proal //et al.// that "1α,25-dihydroxyvitamin D3 exerts tissue-specific effects on estrogen and androgen production and metabolism."(({{pubmed>long:21262387}}))+At normal levels, the active vitamin D metabolite, 1,25-D, serves an important role in host defense,(({{pmid>long:19943126}})) but high levels of the hormone are immunosuppressive(({{pmid>long:19758324}}))  – if for no other reason than the fact that it is calcitriol (1,25-D) and its analogues are used widely to treat autoimmune disease. One of the mechanisms by which 1,25-D may be immunosuppressive (and contribute to symptoms of disease) is by interacting with the body's other nuclear receptors. Selvaraj //et al.// have suggested that the high levels of 1,25-D seen in patients with pulmonary tuberculosis "might lead to downregulation of VDR expression" and that "decreased VDR levels could result in defective VDR signaling."(({{pmid>long:19219539}})) More recently, Johan Lundqvist's 2011 study showed that, consistent with the 2009 study by Proal //et al.// that "1α,25-dihydroxyvitamin D3 exerts tissue-specific effects on estrogen and androgen production and metabolism."(({{pmid>long:21262387}}))
  
-Molecular modeling data show that at high levels, 1,25-D not only binds the VDR but also has a strong affinity for other key receptors that control the body's major hormonal systems including those that regulate the body's sex, thyroid, and adrenal hormones.  As 1,25-D rises, it pushes out the molecules that are meant to control these receptors. Compromising these receptors can disrupt the body's ability to regulate temperature, libido, and any number of other functions.[table of affinities needed] Indeed, in the human brain, the VDR tends to be most common in the hypothalamus, which is responsible for these functions.(({{pubmed>long:15589699}}))+Molecular modeling data show that at high levels, 1,25-D not only binds the VDR but also has a strong affinity for other key receptors that control the body's major hormonal systems including those that regulate the body's sex, thyroid, and adrenal hormones.  As 1,25-D rises, it pushes out the molecules that are meant to control these receptors. Compromising these receptors can disrupt the body's ability to regulate temperature, libido, and any number of other functions.[table of affinities needed] Indeed, in the human brain, the VDR tends to be most common in the hypothalamus, which is responsible for these functions.(({{pmid>long:15589699}}))
  
  
-Molecular research also shows that, like the VDR, several of these nuclear receptors (including the alpha/beta thyroid receptors, glucocorticoid receptor, and androgen receptor) also express many families of antimicrobial peptides. A recent analysis of AmP expression by Brahmachary showed that the glucocorticoid receptor, the androgen receptor, and the Vitamin D Receptor, seem to be in control of 20, 17 and 16 families respectively, out of 22 analyzed.(({{pubmed>long:17254313}}))  This means that when elevated 1,25-D displaces their endogenous ligands, the body's overall AmP expression is thwarted to an even greater degree.  This further impairs the innate immune system's ability to combat chronic pathogens.    +Molecular research also shows that, like the VDR, several of these nuclear receptors (including the alpha/beta thyroid receptors, glucocorticoid receptor, and androgen receptor) also express many families of antimicrobial peptides. A recent analysis of AmP expression by Brahmachary showed that the glucocorticoid receptor, the androgen receptor, and the Vitamin D Receptor, seem to be in control of 20, 17 and 16 families respectively, out of 22 analyzed.(({{pmid>long:17254313}}))  This means that when elevated 1,25-D displaces their endogenous ligands, the body's overall AmP expression is thwarted to an even greater degree.  This further impairs the innate immune system's ability to combat chronic pathogens.    
  
  
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-[{{ :home:alternate:sarcoidosiscathelicidin.001.png?400|**If anything, patients with autoimmune disease are //immunocompromised//.** A study of the prevalence of the key antimicrobial peptide showed that patients with sarcoidosis expressed it less than healthy subjects, and that sicker sarcoidosis patients expressed it least of all. Source: [[http://meeting.chestpubs.org/cgi/content/abstract/136/4/127S-a|Kanchwala et al.]]}}]+[{{ :home:alternate:sarcoidosiscathelicidin.001.png?400|**If anything, patients with autoimmune disease are //immunocompromised//.** A study of the prevalence of the key antimicrobial peptide showed that patients with sarcoidosis expressed it less than healthy subjects, and that sicker sarcoidosis patients expressed it least of all. Source: [[https://meeting.chestpubs.org/cgi/content/abstract/136/4/127S-a|Kanchwala et al.]]}}]
  
 ===== End-stage disease ===== ===== End-stage disease =====
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-As patients become sicker, their immune system becomes increasingly unable to mount a defense against infection. For example, Kanchwala //et al.// showed that patients with sarcoidosis expressed the antimicrobial peptide cathelicidin less than healthy subjects, and that sicker sarcoidosis patients expressed it least of all.((Kanchwala, A., B. Barna, et al. (2009). [[http://meeting.chestpubs.org/cgi/content/abstract/136/4/127S-a|Deficiencies of cathelicidin and vitamin D accompany disease severity in sarcoidosis]].))+As patients become sicker, their immune system becomes increasingly unable to mount a defense against infection. For example, Kanchwala //et al.// showed that patients with sarcoidosis expressed the antimicrobial peptide cathelicidin less than healthy subjects, and that sicker sarcoidosis patients expressed it least of all.((Kanchwala, A., B. Barna, et al. (2009). [[https://meeting.chestpubs.org/cgi/content/abstract/136/4/127S-a|Deficiencies of cathelicidin and vitamin D accompany disease severity in sarcoidosis]].))
  
 One of the hallmarks of late-stage inflammatory diseases is a very low 1,25-D with HIV/AIDS being the most commonly cited example.  One of the hallmarks of late-stage inflammatory diseases is a very low 1,25-D with HIV/AIDS being the most commonly cited example. 
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 HIV is a viral infection, and AIDS is the syndrome, which results – according to the Marshall Pathogenesis – in a dysregulated vitamin D metabolism. As evidenced by the subset of people who survive for decades with HIV, the virus itself is not necessarily deadly. Instead, it is the co-infections which are the proximate cause of the disease. One can define the breadth of AIDS-related complications by the extent and number of co-infections such as pneumonia, herpes, //Candida//, etc. HIV is a viral infection, and AIDS is the syndrome, which results – according to the Marshall Pathogenesis – in a dysregulated vitamin D metabolism. As evidenced by the subset of people who survive for decades with HIV, the virus itself is not necessarily deadly. Instead, it is the co-infections which are the proximate cause of the disease. One can define the breadth of AIDS-related complications by the extent and number of co-infections such as pneumonia, herpes, //Candida//, etc.
  
-Supporting this hypothesis, a number of terminal AIDS patients have neglible levels of 1,25-D. 18 of 29 patients in a study of AIDS patients had undetectable levels of the metabolite.(({{pubmed>long:9814454}})) The patients with depressed levels of 1,25-D were characterized by advanced clinical HIV infection, low CD4+ lymphocyte counts, and high serum levels of tumor TNF-alpha – all indication of more severe forms of the disease. +Supporting this hypothesis, a number of terminal AIDS patients have neglible levels of 1,25-D. 18 of 29 patients in a study of AIDS patients had undetectable levels of the metabolite.(({{pmid>long:9814454}})) The patients with depressed levels of 1,25-D were characterized by advanced clinical HIV infection, low CD4+ lymphocyte counts, and high serum levels of tumor TNF-alpha – all indication of more severe forms of the disease. 
  
 The exact mechanism by which 1,25-D is downregulated is not entirely known, but it is highly likely that it is caused by pathogens.  The exact mechanism by which 1,25-D is downregulated is not entirely known, but it is highly likely that it is caused by pathogens. 
  
-Haug //et al// theorized that TNF-alpha and possibly other cytokines – which pathogens are known to create(({{pubmed>long:16466631}})) – inhibit conversion of 25-D into 1,25-D in late-stage cases of HIV/AIDS.(({{pubmed>long:9814454}}))+Haug //et al// theorized that TNF-alpha and possibly other cytokines – which pathogens are known to create(({{pmid>long:16466631}})) – inhibit conversion of 25-D into 1,25-D in late-stage cases of HIV/AIDS.(({{pmid>long:9814454}}))
  
-A second factor is that HIV disables D-Binding protein (DBP).(({{pubmed>long:16545013}})) (({{pubmed>long:19031451}})) DBP is the precursor for Macrophage Activating Factor (MAF) as it has a key role to play in attracting macrophages to sites of injury. 1,25-D is transported throughout the blood by DBP. +A second factor is that HIV disables D-Binding protein (DBP).(({{pmid>long:16545013}})) (({{pmid>long:19031451}})) DBP is the precursor for Macrophage Activating Factor (MAF) as it has a key role to play in attracting macrophages to sites of injury. 1,25-D is transported throughout the blood by DBP. 
 ==== Cancer ==== ==== Cancer ====
  
 <relatedarticle> [[home:pathogenesis:vitamind:cancer|Vitamin D and cancer]]</article> <relatedarticle> [[home:pathogenesis:vitamind:cancer|Vitamin D and cancer]]</article>
  
-Higher levels of CYP24A1, the enzyme which breaks down excess 1,25-D, is associated with poorer survival in lung adenocarcinoma. In a 2011 study, CYP24A1 mRNA was elevated 8-50 fold in lung cancer (compared to normal non-cancerous lung) and significantly higher in less severe cancers.(({{pubmed>long:21169243}}))+Higher levels of CYP24A1, the enzyme which breaks down excess 1,25-D, is associated with poorer survival in lung adenocarcinoma. In a 2011 study, CYP24A1 mRNA was elevated 8-50 fold in lung cancer (compared to normal non-cancerous lung) and significantly higher in less severe cancers.(({{pmid>long:21169243}}))
  
 Lopes //et al.// showed that CYP24A1 expression was increased in metastatic breast cancer: 53.7% in invasive carcinomas compared to 19.0% of benign lesions. Lopes //et al.// showed that CYP24A1 expression was increased in metastatic breast cancer: 53.7% in invasive carcinomas compared to 19.0% of benign lesions.
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 <blockquote>From this study, we conclude that there is a deregulation of the Vitamin D signalling and metabolic pathways in breast cancer, favouring tumour progression. Thus, during mammary malignant transformation, tumour cells lose their ability to synthesize the active form of Vitamin D and respond to VDR-mediated Vitamin D effects, while increasing their ability to degrade this hormone. <blockquote>From this study, we conclude that there is a deregulation of the Vitamin D signalling and metabolic pathways in breast cancer, favouring tumour progression. Thus, during mammary malignant transformation, tumour cells lose their ability to synthesize the active form of Vitamin D and respond to VDR-mediated Vitamin D effects, while increasing their ability to degrade this hormone.
  
-//**N. Lopes**,(({{pubmed>long:20831823}}))//</blockquote>+//**N. Lopes**,(({{pmid>long:20831823}}))//</blockquote>
 ===== Related publications and presentations ===== ===== Related publications and presentations =====
  
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 {{tag>pathogenesis Science_behind_vitamin_D}} {{tag>pathogenesis Science_behind_vitamin_D}}
 +<nodisp>
 ===== Notes and comments ===== ===== Notes and comments =====
  
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 Description of Jun Sun's work: Description of Jun Sun's work:
-http://www.sciencedaily.com/releases/2010/07/100707141558.htm+https://www.sciencedaily.com/releases/2010/07/100707141558.htm
  
  
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 <blockquote> <blockquote>
-Not sure where this should go. Here? Psoriasis? Innate immunity?+Not sure where this should go. Here? Psoriasis? Innate immunity?  MOVED >Psoriasis
  
 PLoS One. 2009 Jul 22;4(7):e6340. PLoS One. 2009 Jul 22;4(7):e6340.
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 Peric M, Koglin S, Dombrowski Y, Gross K, Bradac E, Büchau A, Steinmeyer A, Zügel U, Ruzicka T, Schauber J. Peric M, Koglin S, Dombrowski Y, Gross K, Bradac E, Büchau A, Steinmeyer A, Zügel U, Ruzicka T, Schauber J.
-Department of Dermatology and Allergology, Ludwig-Maximilians-University, Munich, Germany. 
-Abstract 
-Antimicrobial peptides (AMPs) are strongly expressed in lesional skin in psoriasis and play an important role as proinflammatory "alarmins" in this chronic skin disease. Vitamin D analogs like calcipotriol have antipsoriatic effects and might mediate this effect by changing AMP expression. In this study, keratinocytes in lesional psoriatic plaques showed decreased expression of the AMPs beta-defensin (HBD) 2 and HBD3 after topical treatment with calcipotriol. At the same time, calcipotriol normalized the proinflammatory cytokine milieu and decreased interleukin (IL)-17A, IL-17F and IL-8 transcript abundance in lesional psoriatic skin. In contrast, cathelicidin antimicrobial peptide expression was increased by calcipotriol while psoriasin expression remained unchanged. In cultured human epidermal keratinocytes the effect of different vitamin D analogs on the expression of AMPs was further analyzed. All vitamin D analogs tested blocked IL-17A induced HBD2 expression by increasing IkappaB-alpha protein and inhibition of NF-kappaB signaling. At the same time vitamin D analogs induced cathelicidin through activation of the vitamin D receptor and MEK/ERK signaling. These studies suggest that vitamin D analogs differentially alter AMP expression in lesional psoriatic skin and cultured keratinocytes. Balancing AMP "alarmin" expression might be a novel goal in treatment of chronic inflammatory skin diseases. 
  
 PMID: 19623255 PMID: 19623255
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 For a very long time it has been known that EBV delays cell-death, an obvious way of allowing the microbiota to form stable communities within human cells. Here is a 1991 paper: For a very long time it has been known that EBV delays cell-death, an obvious way of allowing the microbiota to form stable communities within human cells. Here is a 1991 paper:
  
-http://www.ncbi.nlm.nih.gov/pubmed/1659776+https://www.ncbi.nlm.nih.gov/pubmed/1659776
  
 Another early paper showed Sarcoidosis was also associated with reduced cell-death (I met one of the authors of this paper last week at the Microbiome conference, and he was surprised when I told him how important it had been in helping me formulate the early MP pathogenesis): Another early paper showed Sarcoidosis was also associated with reduced cell-death (I met one of the authors of this paper last week at the Microbiome conference, and he was surprised when I told him how important it had been in helping me formulate the early MP pathogenesis):
  
-http://www.smw.ch/docs/pdf200x/2001/31/smw-09808.pdf+https://www.smw.ch/docs/pdf200x/2001/31/smw-09808.pdf
  
 Well, a new paper is out showing that EBV not only down-regulates expression of the VDR protein itself, but also acts to block transcription by the VDR: Well, a new paper is out showing that EBV not only down-regulates expression of the VDR protein itself, but also acts to block transcription by the VDR:
  
-http://www.ncbi.nlm.nih.gov/pubmed/20593215+https://www.ncbi.nlm.nih.gov/pubmed/20593215
  
 Here are some of the key points from the fulltext of this paper: Here are some of the key points from the fulltext of this paper:
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 PMID: 17113648 PMID: 17113648
  
-Vitamin D Binding Protein-Macrophage Activating Factor Directly Inhibits Proliferation, Migration, and uPAR Expression of Prostate Cancer Cells. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2956649/?tool=pubmed</blockquote>+Vitamin D Binding Protein-Macrophage Activating Factor Directly Inhibits Proliferation, Migration, and uPAR Expression of Prostate Cancer Cells. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2956649/?tool=pubmed</blockquote> 
 + 
 +===== References =====</nodisp>
  
-===== References ===== 
home/pathogenesis/vitamind/metabolism.1546385345.txt.gz · Last modified: 01.01.2019 by sallieq
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