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     * **complete blood count (CBC)** – A test panel requested by a doctor or other medical professional that gives information about the cells in a patient's blood. Widely used for regular checkups.     * **complete blood count (CBC)** – A test panel requested by a doctor or other medical professional that gives information about the cells in a patient's blood. Widely used for regular checkups.
       * **[[home:tests:electrolytes|electrolytes]]**       * **[[home:tests:electrolytes|electrolytes]]**
-      * **[[home:tests:glucose|glucose]]** 
       * **kidney function** – //see [[#kidney_renal_function|above]]//       * **kidney function** – //see [[#kidney_renal_function|above]]//
-      * **[[home:tests:lipids|lipid profile]]** – Many observations that conflict with the LDL receptor hypothesis (i.e. high LDL cholesterol is the most important cause of atherosclerosis), may be explained by the idea that high serum cholesterol and/or high LDL is protective against infection and atherosclerosis.(({{pubmed>long:14631060}}))  +      * **[[home:tests:lipids|lipid profile]]** – Many observations that conflict with the LDL receptor hypothesis (i.e. high LDL cholesterol is the most important cause of atherosclerosis), may be explained by the idea that high serum cholesterol and/or high LDL is protective against infection and atherosclerosis.(({{pmid>long:14631060}}))  
-      * **[[home:tests:liver|liver function]]** – +      * **[[home:tests:liver|liver function]]** 
 +      * **[[home:diseases:anemia#tests|red blood cell measures]]** – Low levels of iron or cells that transport iron reflect the body's attempt to limit infection.
       * **other measures of inflammation** – Several other measures may wax and wane with immunopathology including [[home:tests:ACE|ACE (angiotensin-converting enzyme)]], [[home:tests:crp|C-reactive protein]], cytokine levels, and ESR       * **other measures of inflammation** – Several other measures may wax and wane with immunopathology including [[home:tests:ACE|ACE (angiotensin-converting enzyme)]], [[home:tests:crp|C-reactive protein]], cytokine levels, and ESR
       * **[[home:othertreatments:vitamins|vitamins and minerals]]** – [[.:food:calcium|calcium]], [[home:food:folic|folic acid and folate]], and supplemental vitamin D (see [[#vitamin_d_metabolism|above]]) are discussed in separate articles       * **[[home:othertreatments:vitamins|vitamins and minerals]]** – [[.:food:calcium|calcium]], [[home:food:folic|folic acid and folate]], and supplemental vitamin D (see [[#vitamin_d_metabolism|above]]) are discussed in separate articles
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 It is best to always ask for copies of your lab reports, x-rays reports, clinic notes, etc. A proactive patient knows exactly what is in his/her medical record. It is best to always ask for copies of your lab reports, x-rays reports, clinic notes, etc. A proactive patient knows exactly what is in his/her medical record.
  
-{{tag> }}+ 
 + 
 + 
 +===== Excessive testing ===== 
 + 
 +Patients should be wary of excessive testing and procedures. 
 + 
 +{{section>:home:pathogenesis:th1spectrum#excessive_testing&noheader&firstseconly}} 
 + 
 + 
 + 
 +{{tag>tests }} 
 +<nodisp>
 ===== Notes and comments ===== ===== Notes and comments =====
-EDIT+
  
   * Legacy content   * Legacy content
-    * http://www.marshallprotocol.com/forum32/3833.html#p33979 f249 +    * https://www.marshallprotocol.com/forum32/3833.html#p33979 f249 
-    * http://www.marshallprotocol.com/view_topic.php?id=2019&forum_id=32&jump_to=75795#p75795 f215 +    * https://www.marshallprotocol.com/view_topic.php?id=2019&forum_id=32&jump_to=75795#p75795 f215 
-    * http://www.marshallprotocol.com/forum32/4264.html f188 +    * https://www.marshallprotocol.com/forum32/4264.html f188 
-    * http://www.marshallprotocol.com/view_topic.php?id=3833&forum_id=32&jump_to=71683#p71683 f251 +    * https://www.marshallprotocol.com/view_topic.php?id=3833&forum_id=32&jump_to=71683#p71683 f251 
-    * http://www.marshallprotocol.com/forum32/1198.html#p9329 f195 +    * https://www.marshallprotocol.com/forum32/1198.html#p9329 f195 
-    * http://www.marshallprotocol.com/view_topic.php?id=1198&forum_id=32&jump_to=84710#p84710 f197 +    * https://www.marshallprotocol.com/view_topic.php?id=1198&forum_id=32&jump_to=84710#p84710 f197 
-    * http://www.marshallprotocol.com/view_topic.php?id=3479&forum_id=32&jump_to=71473#p71473 f253 +    * https://www.marshallprotocol.com/view_topic.php?id=3479&forum_id=32&jump_to=71473#p71473 f253 
-    * http://www.marshallprotocol.com/forum32/3178.html f317 +    * https://www.marshallprotocol.com/forum32/3178.html f317 
-    * http://www.marshallprotocol.com/view_topic.php?id=7350&forum_id=32&jump_to=100406#p100406 f135 +    * https://www.marshallprotocol.com/view_topic.php?id=7350&forum_id=32&jump_to=100406#p100406 f135 
-    * http://www.marshallprotocol.com/forum32/7350.html#p76298 f132 +    * https://www.marshallprotocol.com/forum32/7350.html#p76298 f132 
-    * http://www.marshallprotocol.com/forum32/8622.html f145 +    * https://www.marshallprotocol.com/forum32/8622.html f145 
-    * http://www.marshallprotocol.com/forum32/7728.html#p82521 f200 +    * https://www.marshallprotocol.com/forum32/7728.html#p82521 f200 
-    * http://www.marshallprotocol.com/forum32/3471.html#p31063 f258 +    * https://www.marshallprotocol.com/forum32/3471.html#p31063 f258 
-    * http://www.marshallprotocol.com/view_topic.php?id=3471&forum_id=32&jump_to=67119#p67119 f259 +    * https://www.marshallprotocol.com/view_topic.php?id=3471&forum_id=32&jump_to=67119#p67119 f259 
-    * http://www.marshallprotocol.com/view_topic.php?id=7728&forum_id=32&jump_to=84635#p84635 f202 +    * https://www.marshallprotocol.com/view_topic.php?id=7728&forum_id=32&jump_to=84635#p84635 f202 
-    * http://www.marshallprotocol.com/view_topic.php?id=3815&forum_id=32&jump_to=75778#p75778 f192 +    * https://www.marshallprotocol.com/view_topic.php?id=3815&forum_id=32&jump_to=75778#p75778 f192 
-    * http://www.marshallprotocol.com/view_topic.php?id=4264&forum_id=32&jump_to=85235#p85235 f190 +    * https://www.marshallprotocol.com/view_topic.php?id=4264&forum_id=32&jump_to=85235#p85235 f190 
-    * http://www.marshallprotocol.com/forum32/5450.html f282 +    * https://www.marshallprotocol.com/forum32/5450.html f282 
-    * http://www.marshallprotocol.com/forum32/4264.html#p69981 f189 +    * https://www.marshallprotocol.com/forum32/4264.html#p69981 f189 
-    * http://www.marshallprotocol.com/view_topic.php?id=10992&forum_id=32&jump_to=133747#p133747 f117 +    * https://www.marshallprotocol.com/view_topic.php?id=10992&forum_id=32&jump_to=133747#p133747 f117 
-    * http://www.marshallprotocol.com/view_topic.php?id=2495&forum_id=32&jump_to=45595#p45595 f229 +    * https://www.marshallprotocol.com/view_topic.php?id=2495&forum_id=32&jump_to=45595#p45595 f229 
-    * http://www.marshallprotocol.com/view_topic.php?id=3833&forum_id=32&jump_to=71474#p71474 f250 +    * https://www.marshallprotocol.com/view_topic.php?id=3833&forum_id=32&jump_to=71474#p71474 f250 
-    * http://www.marshallprotocol.com/forum32/2019.html#p16519 f214 +    * https://www.marshallprotocol.com/forum32/2019.html#p16519 f214 
-    * http://www.marshallprotocol.com/forum32/1200.html f343 +    * https://www.marshallprotocol.com/forum32/1200.html f343 
-    * http://www.marshallprotocol.com/forum32/1599.html f338 +    * https://www.marshallprotocol.com/forum32/1599.html f338 
-    * http://www.marshallprotocol.com/view_topic.php?id=2019&forum_id=32&jump_to=81508#p81508 f216 +    * https://www.marshallprotocol.com/view_topic.php?id=2019&forum_id=32&jump_to=81508#p81508 f216 
-    * http://www.marshallprotocol.com/forum32/2880.html f321 +    * https://www.marshallprotocol.com/forum32/2880.html f321 
-    * http://www.marshallprotocol.com/forum32/2155.html f326 +    * https://www.marshallprotocol.com/forum32/2155.html f326 
-    * http://www.marshallprotocol.com/forum32/2487.html#p21648  f106  +    * https://www.marshallprotocol.com/forum32/2487.html#p21648  f106  
-    * http://www.marshallprotocol.com/view_topic.php? id=2487&forum_id=32&jump_to=90167#p90167  f110 +    * https://www.marshallprotocol.com/view_topic.php? id=2487&forum_id=32&jump_to=90167#p90167  f110 
     *      * 
  
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-Total Protein test is [[http://labtestsonline.org/understanding/analytes/tp/test.html|described here]].+Total Protein test is [[https://labtestsonline.org/understanding/analytes/tp/test.html|described here]].
  
-Albumin test is [[http://www.labtestsonline.org/understanding/analytes/albumin/test.html|describedhere]].+Albumin test is [[https://www.labtestsonline.org/understanding/analytes/albumin/test.html|describedhere]].
  
-Calcium test is [[http://www.labtestsonline.org/understanding/analytes/calcium/test.html|describedhere]].+Calcium test is [[https://www.labtestsonline.org/understanding/analytes/calcium/test.html|describedhere]].
  
-Alkaline Phosphatase test is [[http://www.labtestsonline.org/understanding/analytes/alp/test.html|describedhere]].+Alkaline Phosphatase test is [[https://www.labtestsonline.org/understanding/analytes/alp/test.html|describedhere]].
  
-Aspartate Aminotransferase test is [[http://www.labtestsonline.org/understanding/analytes/ast/test.html|describedhere]].+Aspartate Aminotransferase test is [[https://www.labtestsonline.org/understanding/analytes/ast/test.html|describedhere]].
  
 Serum glutamic-oxaloacetic transaminase, SGOT Serum glutamic-oxaloacetic transaminase, SGOT
  
-Bilirubin test is [[http://www.labtestsonline.org/understanding/analytes/bilirubin/test.html|describedhere]].+Bilirubin test is [[https://www.labtestsonline.org/understanding/analytes/bilirubin/test.html|describedhere]].
  
-CBC (complete blood count) with differential test is [[http://labtestsonline.org/understanding/analytes/cbc/test.html|described here]].+CBC (complete blood count) with differential test is [[https://labtestsonline.org/understanding/analytes/cbc/test.html|described here]].
  
     * basophils     * basophils
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-The cortisol test is [[http://labtestsonline.org/understanding/analytes/cortisol/test.html|described here]].+The cortisol test is [[https://labtestsonline.org/understanding/analytes/cortisol/test.html|described here]].
  
-C-peptide test is [[http://labtestsonline.org/understanding/analytes/c_peptide/test.html|described here]].+C-peptide test is [[https://labtestsonline.org/understanding/analytes/c_peptide/test.html|described here]].
  
-Gamma-Glutamyl Transferase (GGT) test is [[http://labtestsonline.org/understanding/analytes/ggt/test.html|described here]].+Gamma-Glutamyl Transferase (GGT) test is [[https://labtestsonline.org/understanding/analytes/ggt/test.html|described here]].
  
-Lipase test is [[http://labtestsonline.org/understanding/analytes/lipase/test.html|described here]].+Lipase test is [[https://labtestsonline.org/understanding/analytes/lipase/test.html|described here]].
  
  
-Insulin test is [[http://labtestsonline.org/understanding/analytes/insulin/test.html|described here]].+Insulin test is [[https://labtestsonline.org/understanding/analytes/insulin/test.html|described here]].
  
  
-Phosphorous test is [[http://labtestsonline.org/understanding/analytes/phosphorus/test.html|described here]].+Phosphorous test is [[https://labtestsonline.org/understanding/analytes/phosphorus/test.html|described here]].
  
  
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 </blockquote> </blockquote>
  
-[[http://en.wikipedia.org/wiki/Reference_ranges_for_blood_tests|Wikipedia's reference range for blood tests]]+[[https://en.wikipedia.org/wiki/Reference_ranges_for_blood_tests|Wikipedia's reference range for blood tests]]
  
  
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 **Phillyguy** **Phillyguy**
  
-http://dspace.creighton.edu/xmlui/handle/10504/7063+https://dspace.creighton.edu/xmlui/handle/10504/7063
  
  
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 Asthma is one of the most chronic medical conditions characterized by inflammation of the airways, mucous production, and airway hyperresponsiveness. A hallmark of asthma is the presence and activation of inflammatory cells in the airways, notably eosinophils, mast cells and T lymphocytes. Eosinophils function is related to destructive activity mediated by the toxic effects of the granule proteins and soluble mediators derived by the cells. Vitamin D, as a potent immunomodulator, has an unknown role in the eosinophil-induced asthmatic response. It was previously reported that in vivo administration of vitamin D in humans increases the expression of CC chemokine receptor 3 (CCR3) in eosinophils. However, whether or not the effect of vitamin D on eosinophils is direct or indirect is unclear. Calcitriol, the active form of vitamin D, can induce synthesis of Transforming Growth Factor (TGF-β), which is involved in airway remodeling. Eosinophils, which are recruited to the lungs by eotaxin and interleukin 5 (IL-5), are a major source of TGF-β. Therefore, the effect of calcitriol was examined on vitamin D receptor (VDR), CCR3, TGF-β1, and IL-5 receptor (IL-5R), expression in human blood eosinophils. Peripheral blood was drawn from healthy volunteers, and eosinophils, neutrophils, monocytes and lymphocytes were isolated. The purified eosinophils cells (purity >99%; viability >97%) were stimulated with calcitriol (100-300 pM and 100 nM) for 24 hours. The mRNA expression and protein expression of CCR3, VDR, TGF-β1, and IL-5R was examined by real-time PCR and Western blotting. Eosinophil chemotaxis was also assessed using Transwell migration assay and using eotaxin and IL-5 as chemokines. Also, the role of GATA-1which is a member of GATA transcription factor family involved in eosinophil development was examined using siRNA. After calcitriol stimulation of eosinophils, VDR mRNA and protein expression were increased significantly. Eosinophils also had a significant increase in CCR3 mRNA and protein expression of eosinophils as well as a significant increase in mRNA and protein expression of TGF-β1. However, Calcitriol had no effect on IL-5R mRNA transcripts in eosinophils. Calcitriol was found to significantly enhance the migration of eosinophils in response to eotaxin, but it had no effect on the migration of eosinophils in response to IL-5. Calcitriol induced phosphorylation and increase protein expression of GATA-1. siRNA specific for GATA -1 reduced calcitriol-induced phosphorylation and increased GATA-1 protein expression, in addition, calcitriol increased protein levels of CCR3. These results demonstrate that vitamin D can directly act on human blood eosinophils to increase mRNA transcripts of CCR3, TGF-β1 and enhancing migration of eosinophils in response to eotaxin. This suggests a potential role of vitamin D in eosinophil recruitment and migration in inflammatory airways.</blockquote> Asthma is one of the most chronic medical conditions characterized by inflammation of the airways, mucous production, and airway hyperresponsiveness. A hallmark of asthma is the presence and activation of inflammatory cells in the airways, notably eosinophils, mast cells and T lymphocytes. Eosinophils function is related to destructive activity mediated by the toxic effects of the granule proteins and soluble mediators derived by the cells. Vitamin D, as a potent immunomodulator, has an unknown role in the eosinophil-induced asthmatic response. It was previously reported that in vivo administration of vitamin D in humans increases the expression of CC chemokine receptor 3 (CCR3) in eosinophils. However, whether or not the effect of vitamin D on eosinophils is direct or indirect is unclear. Calcitriol, the active form of vitamin D, can induce synthesis of Transforming Growth Factor (TGF-β), which is involved in airway remodeling. Eosinophils, which are recruited to the lungs by eotaxin and interleukin 5 (IL-5), are a major source of TGF-β. Therefore, the effect of calcitriol was examined on vitamin D receptor (VDR), CCR3, TGF-β1, and IL-5 receptor (IL-5R), expression in human blood eosinophils. Peripheral blood was drawn from healthy volunteers, and eosinophils, neutrophils, monocytes and lymphocytes were isolated. The purified eosinophils cells (purity >99%; viability >97%) were stimulated with calcitriol (100-300 pM and 100 nM) for 24 hours. The mRNA expression and protein expression of CCR3, VDR, TGF-β1, and IL-5R was examined by real-time PCR and Western blotting. Eosinophil chemotaxis was also assessed using Transwell migration assay and using eotaxin and IL-5 as chemokines. Also, the role of GATA-1which is a member of GATA transcription factor family involved in eosinophil development was examined using siRNA. After calcitriol stimulation of eosinophils, VDR mRNA and protein expression were increased significantly. Eosinophils also had a significant increase in CCR3 mRNA and protein expression of eosinophils as well as a significant increase in mRNA and protein expression of TGF-β1. However, Calcitriol had no effect on IL-5R mRNA transcripts in eosinophils. Calcitriol was found to significantly enhance the migration of eosinophils in response to eotaxin, but it had no effect on the migration of eosinophils in response to IL-5. Calcitriol induced phosphorylation and increase protein expression of GATA-1. siRNA specific for GATA -1 reduced calcitriol-induced phosphorylation and increased GATA-1 protein expression, in addition, calcitriol increased protein levels of CCR3. These results demonstrate that vitamin D can directly act on human blood eosinophils to increase mRNA transcripts of CCR3, TGF-β1 and enhancing migration of eosinophils in response to eotaxin. This suggests a potential role of vitamin D in eosinophil recruitment and migration in inflammatory airways.</blockquote>
  
-===== References =====+ 
 +<blockquote>My own Doc wanted me to have a biopsy after my PSA doubled in 12 months at an early point in MP recovery... I dissuaded him by citing the European guidelines, which are much more conservative. Anyway, here is something we might want to help Paul put in the MPKB :) Maybe we also want the European guidelines in there too :) 
 +https://www.businessweek.com/lifestyle/content/healthday/650257.html..trevor.. 
 +</blockquote> 
 + 
 +Is triglycerides any good indicator of immmuopathology? I suggest this is removed. Inge Lindseth 
 + 
 +===== References =====</nodisp> 
home/tests.txt · Last modified: 09.14.2022 by 127.0.0.1
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