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home:pathogenesis:microbiota:biofilm [01.11.2019] – [More research] sallieq | home:pathogenesis:microbiota:biofilm [09.14.2022] (current) – external edit 127.0.0.1 | ||
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====== Biofilm bacteria | ====== Biofilm bacteria | ||
- | Biofilms are densely packed communities of microbial cells that grow on living or inert surfaces and surround themselves with secreted polymers. Many bacterial species form biofilms, and their study has revealed them to be complex and diverse. The structural and physiological complexity of biofilms has led to the idea that they are coordinated and cooperative groups, analogous to multicellular organisms.(({{pubmed> | + | Biofilms are densely packed communities of microbial cells that grow on living or inert surfaces and surround themselves with secreted polymers. Many bacterial species form biofilms, and their study has revealed them to be complex and diverse. The structural and physiological complexity of biofilms has led to the idea that they are coordinated and cooperative groups, analogous to multicellular organisms.(({{pmid> |
Researchers have estimated that 60-80 percent of microbial infections in the body are caused by bacteria growing as a biofilm – as opposed to planktonic (free-floating) bacteria. | Researchers have estimated that 60-80 percent of microbial infections in the body are caused by bacteria growing as a biofilm – as opposed to planktonic (free-floating) bacteria. | ||
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- | Biofilm-related disease. (({{pubmed> | + | Biofilm-related disease. (({{pmid> |
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- | In the years which followed, researchers have concentrated primarily on planktonic (free-floating) bacteria, the kinds of microbes studied by the likes of Louis Pasteur and Robert Koch. It was not until the 1970s that scientists began to appreciate that bacteria in the biofilm mode of existence constitute such a major component of the bacterial biomass in most environments. In the 1980s and 1990s, scientists began to understand how elaborately organized a bacterial biofilm community can be.(({{pubmed> | + | In the years which followed, researchers have concentrated primarily on planktonic (free-floating) bacteria, the kinds of microbes studied by the likes of Louis Pasteur and Robert Koch. It was not until the 1970s that scientists began to appreciate that bacteria in the biofilm mode of existence constitute such a major component of the bacterial biomass in most environments. In the 1980s and 1990s, scientists began to understand how elaborately organized a bacterial biofilm community can be.(({{pmid> |
- | Paul Stoodley of the Center for Biofilm Engineering at Montana State University, attributes much of the lag in studying biofilms to the difficulties of working with heterogeneous biofilms compared with homogeneous planktonic populations. In a 2004 paper in //Nature Reviews//, the molecular biologist describes many reasons why biofilms are extremely difficult to culture, such as the fact that the diffusion of liquid through a biofilm and the fluid forces acting on a biofilm must be carefully calculated if it is to be cultured correctly. According to Stoodley, the need to master such difficult laboratory techniques has deterred many scientists from attempting to work with biofilms.(({{pubmed> | + | Paul Stoodley of the Center for Biofilm Engineering at Montana State University, attributes much of the lag in studying biofilms to the difficulties of working with heterogeneous biofilms compared with homogeneous planktonic populations. In a 2004 paper in //Nature Reviews//, the molecular biologist describes many reasons why biofilms are extremely difficult to culture, such as the fact that the diffusion of liquid through a biofilm and the fluid forces acting on a biofilm must be carefully calculated if it is to be cultured correctly. According to Stoodley, the need to master such difficult laboratory techniques has deterred many scientists from attempting to work with biofilms.(({{pmid> |
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< | < | ||
- | //**Kim Lewis**// (({{pubmed> | + | //**Kim Lewis**// (({{pmid> |
==== Diseases for which biofilm have been implicated ==== | ==== Diseases for which biofilm have been implicated ==== | ||
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In just a short period of time, researchers studying internal biofilms have already determined they cause a number of chronic infections and diseases. Notable diseases include: | In just a short period of time, researchers studying internal biofilms have already determined they cause a number of chronic infections and diseases. Notable diseases include: | ||
- | * **atherosclerosis** – Biofilm //may// contribute to the development of atherosclerosis. Ott //et al.//' | + | * **atherosclerosis** – Biofilm //may// contribute to the development of atherosclerosis. Ott //et al.//' |
- | * **chronic sinusitis** – One study found that biofilms are present on the removed tissue of two-thirds of patients undergoing surgery for chronic inflammation of the sinuses.(({{pubmed> | + | * **chronic sinusitis** – One study found that biofilms are present on the removed tissue of two-thirds of patients undergoing surgery for chronic inflammation of the sinuses.(({{pmid> |
- | * **chronic wounds** – Biofilm have been implicated in chronic wounds. Dr. Randall Wolcott has published work offering strategies for managing wounds.(({{pubmed> | + | * **chronic wounds** – Biofilm have been implicated in chronic wounds. Dr. Randall Wolcott has published work offering strategies for managing wounds.(({{pmid> |
< | < | ||
- | // | + | // |
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[{{ : | [{{ : | ||
- | * **cystic fibrosis** – The lungs of individuals with cystic fibrosis are colonized and infected by bacteria from an early age. These bacteria, which often spread amongst individuals with CF, thrive in the altered mucus, which collects in the small airways of the lungs. Over time, both the types of bacteria and their individual characteristics change in individuals with CF. In the initial stage, common bacteria such as // | + | * **cystic fibrosis** – The lungs of individuals with cystic fibrosis are colonized and infected by bacteria from an early age. These bacteria, which often spread amongst individuals with CF, thrive in the altered mucus, which collects in the small airways of the lungs. Over time, both the types of bacteria and their individual characteristics change in individuals with CF. In the initial stage, common bacteria such as // |
- | * **endocarditis** – Inflammation of the smooth membranes which line the inside of the heart is caused by a complex biofilm composed of both bacterial and host components.(({{pubmed> | + | * **endocarditis** – Inflammation of the smooth membranes which line the inside of the heart is caused by a complex biofilm composed of both bacterial and host components.(({{pmid> |
- | * **inner ear infections** – The majority of ear infections are caused by biofilm bacteria.(({{pubmed> | + | * **inner ear infections** – The majority of ear infections are caused by biofilm bacteria.(({{pmid> |
< | < | ||
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// | // | ||
- | * **kidney stones** – Biofilms also cause the formation of kidney stones.(({{pubmed> | + | * **kidney stones** – Biofilms also cause the formation of kidney stones.(({{pmid> |
- | * **leptospirosis** – Biofilms also cause leptospirosis, | + | * **leptospirosis** – Biofilms also cause leptospirosis, |
- | * **osteomyelitis** – According to Parsek, biofilms may also cause osteomyelitis, | + | * **osteomyelitis** – According to Parsek, biofilms may also cause osteomyelitis, |
- | * **osteonecrosis and osteomyelitis of the jaw** – Of 20 patients with these bone disease, all " | + | * **osteonecrosis and osteomyelitis of the jaw** – Of 20 patients with these bone disease, all " |
- | [{{ : | + | [{{ : |
* **periodontal disease** – Perhaps the most well-known and studied biofilm bacteria. Hundreds of microbial biofilm colonize the human mouth, causing tooth decay and gum disease. | * **periodontal disease** – Perhaps the most well-known and studied biofilm bacteria. Hundreds of microbial biofilm colonize the human mouth, causing tooth decay and gum disease. | ||
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- | //**Matthew Parsek, PhD**// (({{pubmed> | + | //**Matthew Parsek, PhD**// (({{pmid> |
- | Dental plaque is composed of more than 500 species.(({{pubmed> | + | Dental plaque is composed of more than 500 species.(({{pmid> |
- | * **prosthetic joints and heart valves** – Pathogenic biofims are also commonly found on medical devices such as joint prostheses and heart valves.(({{pubmed> | + | * **prosthetic joints and heart valves** – Pathogenic biofims are also commonly found on medical devices such as joint prostheses and heart valves.(({{pmid> |
< | < | ||
- | // | + | // |
</ | </ | ||
- | * **urinary tract infections** – In their 2003 //Science// paper, Anderson //et al.// reported that in the case of UTIs, intracellular // | + | * **urinary tract infections** – In their 2003 //Science// paper, Anderson //et al.// reported that in the case of UTIs, intracellular // |
- | * **veterinary diseases** – Biofilms have also been implicated in a wide array of veterinary diseases.(({{pubmed> | + | * **veterinary diseases** – Biofilms have also been implicated in a wide array of veterinary diseases.(({{pmid> |
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==== Drinking water ==== | ==== Drinking water ==== | ||
- | According to a 2011 review, biofilms in drinking water systems can serve as a significant environmental reservoir for pathogenic microorganisms.(({{pubmed> | + | According to a 2011 review, biofilms in drinking water systems can serve as a significant environmental reservoir for pathogenic microorganisms.(({{pmid> |
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[{{ : | [{{ : | ||
==== Attachment/ | ==== Attachment/ | ||
- | Biofilms form when bacteria adhere to surfaces in aqueous environments and begin to excrete a slimy, glue-like substance that can anchor them to a variety of materials including metals, plastics, soil particles, medical implant materials and, most significantly, | + | Biofilms form when bacteria adhere to surfaces in aqueous environments and begin to excrete a slimy, glue-like substance that can anchor them to a variety of materials including metals, plastics, soil particles, medical implant materials and, most significantly, |
- | These bacterial pioneers facilitate the arrival of other pathogens by providing more diverse adhesion sites. They also begin to build the matrix that holds the biofilm together. If there are species that are unable to attach to a surface on their own, they are often able to anchor themselves to the matrix or directly to earlier colonists. The expression of 800 genes have been shown to be altered when a single bacterial species joins a biofilm.(({{pubmed> | + | These bacterial pioneers facilitate the arrival of other pathogens by providing more diverse adhesion sites. They also begin to build the matrix that holds the biofilm together. If there are species that are unable to attach to a surface on their own, they are often able to anchor themselves to the matrix or directly to earlier colonists. The expression of 800 genes have been shown to be altered when a single bacterial species joins a biofilm.(({{pmid> |
According to Costerton, the genes that allow a biofilm to develop are activated after enough cells attach to a solid surface. | According to Costerton, the genes that allow a biofilm to develop are activated after enough cells attach to a solid surface. | ||
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< | < | ||
- | //**William Costerton** et al.// (({{pubmed> | + | //**William Costerton** et al.// (({{pmid> |
Research on the molecular and genetic basis of biofilm development has shown that when cells switch from planktonic to community mode, they also undergo a shift in behavior that involves alterations in the activity of numerous genes. There is evidence that specific genes must be transcribed during the attachment phase of biofilm development. In many cases, the activation of these genes is required for synthesis of the extracellular matrix that protects the pathogens inside. | Research on the molecular and genetic basis of biofilm development has shown that when cells switch from planktonic to community mode, they also undergo a shift in behavior that involves alterations in the activity of numerous genes. There is evidence that specific genes must be transcribed during the attachment phase of biofilm development. In many cases, the activation of these genes is required for synthesis of the extracellular matrix that protects the pathogens inside. | ||
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=== Movement === | === Movement === | ||
- | Biofilm bacteria can move in numerous ways that allow them to easily infect new tissues. Biofilms may move collectively, | + | Biofilm bacteria can move in numerous ways that allow them to easily infect new tissues. Biofilms may move collectively, |
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- | Researchers often note that, once biofilms are established, | + | Researchers often note that, once biofilms are established, |
But if planktonic bacteria are periodically released from the biofilms, each time single bacterial forms enter the tissues, the immune system suddenly becomes aware of their presence. It may proceed to mount an inflammatory response that leads to heightened disease symptoms. Thus, the periodic release of planktonic bacteria from some biofilms may be what causes many chronic relapsing infections. | But if planktonic bacteria are periodically released from the biofilms, each time single bacterial forms enter the tissues, the immune system suddenly becomes aware of their presence. It may proceed to mount an inflammatory response that leads to heightened disease symptoms. Thus, the periodic release of planktonic bacteria from some biofilms may be what causes many chronic relapsing infections. | ||
- | As Matthew R. Parsek of Northwestern University describes in a 2003 paper in the Annual Review of Microbiology, | + | As Matthew R. Parsek of Northwestern University describes in a 2003 paper in the Annual Review of Microbiology, |
Parsek believes that the optimal way for bacteria to survive under such circumstances is in a biofilm, stating that “Increasing evidence suggests that the biofilm mode of growth may play a key role in both of these adaptations. Biofilm growth increases the resistance of bacteria to killing and may make organisms less conspicuous to the immune system.... ultimately this moderation of virulence may serve the bacteria’s interest by increasing the longevity of the host.” | Parsek believes that the optimal way for bacteria to survive under such circumstances is in a biofilm, stating that “Increasing evidence suggests that the biofilm mode of growth may play a key role in both of these adaptations. Biofilm growth increases the resistance of bacteria to killing and may make organisms less conspicuous to the immune system.... ultimately this moderation of virulence may serve the bacteria’s interest by increasing the longevity of the host.” | ||
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==== Ability to enter into latent states during inhospitable conditions ==== | ==== Ability to enter into latent states during inhospitable conditions ==== | ||
- | In the midst of inhospitable conditions such as nutrient starvation, microbes in biofilm communities can enter into a viable but nonculturable state.(({{pubmed> | + | In the midst of inhospitable conditions such as nutrient starvation, microbes in biofilm communities can enter into a viable but nonculturable state.(({{pmid> |
==== Freeloaders ==== | ==== Freeloaders ==== | ||
Even though a biofilm tends to benefit all its members, understanding how such cooperation among pathogens evolves and is maintained may represent one of evolutionary biology’s thorniest problems. Biofilm bacteria appear to resolve the problem of freeloaders in at least two ways: | Even though a biofilm tends to benefit all its members, understanding how such cooperation among pathogens evolves and is maintained may represent one of evolutionary biology’s thorniest problems. Biofilm bacteria appear to resolve the problem of freeloaders in at least two ways: | ||
- | * **Increasing species diversity** – Once inside a biofilm, //P. fluorescens//, | + | * **Increasing species diversity** – Once inside a biofilm, //P. fluorescens//, |
* **Disbanding when there are too many freeloaders** | * **Disbanding when there are too many freeloaders** | ||
- | A study of a cultured //E. coli// colony (not necessarily in a biofilm state) found that individual microbes can act altruistically through a form of kin selection.(({{pubmed> | + | A study of a cultured //E. coli// colony (not necessarily in a biofilm state) found that individual microbes can act altruistically through a form of kin selection.(({{pmid> |
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- | The bacteria that become part of a biofilm engage in quorum sensing, a type of decision-making process in which behavior is coordinated through a " | + | The bacteria that become part of a biofilm engage in quorum sensing, a type of decision-making process in which behavior is coordinated through a " |
Quorum sensing can occur within a single bacterial species as well as between diverse species, and can regulate a host of different processes, essentially serving as a simple communication network. A variety of different molecules can be used as signals. | Quorum sensing can occur within a single bacterial species as well as between diverse species, and can regulate a host of different processes, essentially serving as a simple communication network. A variety of different molecules can be used as signals. | ||
- | For example, researchers at the University of Iowa (several of whom are now at the University of Washington) have spent the last decade identifying the molecules that allow the bacterial species //P. aeruginosa// | + | For example, researchers at the University of Iowa (several of whom are now at the University of Washington) have spent the last decade identifying the molecules that allow the bacterial species //P. aeruginosa// |
Singh and his colleagues finally discovered that //P. aeruginosa// | Singh and his colleagues finally discovered that //P. aeruginosa// | ||
- | In a 2000 study published in //Nature//, Singh and colleagues developed a sensitive test which shows //P. aeruginosa// | + | In a 2000 study published in //Nature//, Singh and colleagues developed a sensitive test which shows //P. aeruginosa// |
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===== Olmesartan against biofilm formation ===== | ===== Olmesartan against biofilm formation ===== | ||
- | Olmesartan' | + | Olmesartan' |
===== Images and illustrations of biofilm bacteria ===== | ===== Images and illustrations of biofilm bacteria ===== | ||
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===== More research ===== | ===== More research ===== | ||
- | [[https:// | + | [[https:// |
- | Biofilms and chronic infections (({{pubmed> | + | Biofilms and chronic infections (({{pmid> |
- | From Koch's postulates to biofilm theory. The lesson of Bill Costerton. (({{pubmed> | + | From Koch's postulates to biofilm theory. The lesson of Bill Costerton. (({{pmid> |
- | A specific response to pH by isolates of Haemophilus influenzae | + | A specific response to pH by isolates of Haemophilus influenzae |
- | Wound Biofilm: Current Perspectives and Strategies on Biofilm Disruption and Treatments (({{pubmed> | + | 2017 Wound Biofilm: Current Perspectives and Strategies on Biofilm Disruption and Treatments (({{pmid> |
- | Proteinase K treatment with antibiotics showed a synergistic effect against S. aureus biofilms. | + | 2017 Proteinase K treatment with antibiotics showed a synergistic effect against S. aureus biofilms. |
+ | |||
+ | The role of extracellular DNA in the maintenance of biofilms (({{pmid> | ||
+ | |||
+ | Increased intestinal permeability as a direct effect of treatment with a bacteriophage cocktail | ||
+ | |||
+ | 2017 Novel insights into the role of bacteriophages as potentially pathogenic for mammals | ||
===== Read more ===== | ===== Read more ===== | ||
- | * [[http:// | + | * [[https:// |
- | * [[http:// | + | * [[https:// |
- | * [[http:// | + | * [[https:// |
* [[https:// | * [[https:// | ||
< | < | ||
- | <object width=" | + | <object width=" |
</ | </ | ||
{{tag> | {{tag> | ||
+ | < | ||
===== Notes and comments ===== | ===== Notes and comments ===== | ||
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- | http:// | + | https:// |
Slimy mats of bacteria called biofilms may be the most liquid-repellent materials in nature, researchers have discovered.</ | Slimy mats of bacteria called biofilms may be the most liquid-repellent materials in nature, researchers have discovered.</ | ||
- | [[http:// | + | [[https:// |
+ | ===== References =====</ | ||
- | ===== References ===== |