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home:tests:25d [04.10.2011] – [Sometimes 25-D levels are contrary to expectation] paulalberthome:tests:25d [03.23.2016] – [Units of measurement] sallieq
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-MP patients getting their 25-D levels tested should ensure the [[home:tests:dtesting|relevant instructions]] are closely followed including the freezing of blood samples.+MP patients getting their 25-D levels tested should ensure the [[home:tests:dtesting|relevant instructions]] are closely followed.
  
 ===== Interpreting a 25-D test ===== ===== Interpreting a 25-D test =====
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 ==== Units of measurement ==== ==== Units of measurement ====
-On the Marshall Protocol study site, 25-D levels are typically discussed using ng/ml units rather than pmol/L. The ratio between the two units is: 2.4 ng/ml = 1 nmol/L. To convert nmol/L into ng/ml, multiply by 0.40, as you see in this example:+On the Marshall Protocol study site, 25-D levels are typically discussed using ng/ml units rather than pmol/L. The ratio between the two units is: 1 ng/ml = 2.5 nmol/L. To convert nmol/L into ng/ml, multiply by 0.40, as you see in this example:
  
   60 nmol/L * 0.40 = 24 ng/mL   60 nmol/L * 0.40 = 24 ng/mL
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   * **Minocycline could be activating other receptors** – Another explanation is that the MP itself, through activation of the Pregnane X Nuclear Receptor (PXR) is contributing to higher than normal levels of vitamin D. Marshall Protocol patients take minocycline, which [[home:protocol:mp_antibiotics#activation_of_pregnane_x_nuclear_receptor_minocycline|appears to bind the PXR]]. A similar action [[home:protocol:olmesartan#pregnane_x_receptor_pxr_agonist|may be generated]] by olmesartan (Benicar).  When active, the PXR transcribes the enzyme CYP3A4, which breaks down 1,25-D. When 1,25-D is broken down, 25-D levels escalate. An activated PXR could explain why some MP patients have stubbornly high levels of 25-D even while being extremely careful to avoid consumption of vitamin D.   * **Minocycline could be activating other receptors** – Another explanation is that the MP itself, through activation of the Pregnane X Nuclear Receptor (PXR) is contributing to higher than normal levels of vitamin D. Marshall Protocol patients take minocycline, which [[home:protocol:mp_antibiotics#activation_of_pregnane_x_nuclear_receptor_minocycline|appears to bind the PXR]]. A similar action [[home:protocol:olmesartan#pregnane_x_receptor_pxr_agonist|may be generated]] by olmesartan (Benicar).  When active, the PXR transcribes the enzyme CYP3A4, which breaks down 1,25-D. When 1,25-D is broken down, 25-D levels escalate. An activated PXR could explain why some MP patients have stubbornly high levels of 25-D even while being extremely careful to avoid consumption of vitamin D.
 +  * **Serum levels of 25-D may not be accurate reflection of cellular levels**
  
 +<blockquote>As I have indicated in the past I have seen marked changes in vitamin D serum metabolite measurements including 25-D levels that had nothing to do with diet or light exposure. I have also witnessed immunopathology is patients with serum 25-D levels as high as 30 ng/ml. So obviously what is happening intracellularly does not always reflect in extracellular markers.
  
 +//**Greg Blaney, M.D.**// 
 +</blockquote>
  
 ===== 25-D after recovery ===== ===== 25-D after recovery =====
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 //**Trevor Marshall, PhD**//</blockquote> //**Trevor Marshall, PhD**//</blockquote>
 +
 +
 +===== Patients experiences =====
 +
 +
 +<blockquote>My feeling worse corresponded to dropping levels of 25-D. Although our "therapeutic range" is <12ng/ml, my own experience
 +of going from 12ish to 4ng fairly rapidly was feeling considerably worse pretty  quickly. My own apparent deterioration seems to have begun to reverse over the subsequent 8-9 months. Everyone's different, but perhaps you'll also find this to be the case. So hang in there, and in the meantime do what you can to keep things tolerable.
 +
 +//**pgeek**, MarshallProtocol.com//
 +</blockquote>
 +
  
  
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 ===== Notes and comments ===== ===== Notes and comments =====
-TECHEDIT + 
- +  * Vitamin D: More May Not Be Better; Benefits in Healthy Adults Wear Off at Higher Doses, Research Suggests ... http://www.sciencedaily.com/releases/2013/05/130501192929.htm ...  
 +"Above 21 nanograms per milliliters, the data suggest that the protective effect appears to wear off" 
   * Need to find page to put link to for the d-metabolite feedback diagram.   * Need to find page to put link to for the d-metabolite feedback diagram.
   * Legacy content    * Legacy content 
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 Roux-en-y gastric bypass (RYGB) surgery is associated with dramatic improvements in obesity-related comorbidity, but also with nutritional deficiencies. Vitamin D concentrations are depressed in the severely obese, but the impact of weight loss via RYGB is unknown. We determined associations between adiposity and systemic 25-hydroxyvitamin D (25(OH)D) during weight loss and the immediate and longer-term effects of RYGB. Plasma 25(OH)D concentrations and fat mass (FAT) were determined by immunoassay and air displacement plethysmography, respectively, at 0 (before RYGB surgery), and at 1, 6, and 24 months in severely obese white and African American (AA) women (n = 20). Decreases in adiposity were observed at 1, 6, and 24 months following RYGB (P < 0.05). Plasma 25(OH)D concentrations increased at 1 month (P = 0.004); a decreasing trend occurred over the remainder months after surgery (P = 0.02). Despite temporary improvement in vitamin D status, a high prevalence of vitamin D insufficiency was observed (76, 71, 67, and 82%, at baseline, 1, 6, and 24 months, respectively), and plasma 25(OH)D concentrations were lower in AA compared to white patients (P < 0.05). Strong positive baseline and 1 month cross-sectional correlations between FAT and plasma 25(OH)D were observed, which remained after adjustment for age and race subgroup (β = 0.76 and 0.61, respectively, P = 0.02). In conclusion, 25(OH)D concentrations increased temporarily and then decreased during the 24 months following RYGB. The acute increase and the positive associations observed between adipose tissue mass and systemic 25(OH)D concentrations suggest storage in adipose tissue and release during weight loss</blockquote>. Roux-en-y gastric bypass (RYGB) surgery is associated with dramatic improvements in obesity-related comorbidity, but also with nutritional deficiencies. Vitamin D concentrations are depressed in the severely obese, but the impact of weight loss via RYGB is unknown. We determined associations between adiposity and systemic 25-hydroxyvitamin D (25(OH)D) during weight loss and the immediate and longer-term effects of RYGB. Plasma 25(OH)D concentrations and fat mass (FAT) were determined by immunoassay and air displacement plethysmography, respectively, at 0 (before RYGB surgery), and at 1, 6, and 24 months in severely obese white and African American (AA) women (n = 20). Decreases in adiposity were observed at 1, 6, and 24 months following RYGB (P < 0.05). Plasma 25(OH)D concentrations increased at 1 month (P = 0.004); a decreasing trend occurred over the remainder months after surgery (P = 0.02). Despite temporary improvement in vitamin D status, a high prevalence of vitamin D insufficiency was observed (76, 71, 67, and 82%, at baseline, 1, 6, and 24 months, respectively), and plasma 25(OH)D concentrations were lower in AA compared to white patients (P < 0.05). Strong positive baseline and 1 month cross-sectional correlations between FAT and plasma 25(OH)D were observed, which remained after adjustment for age and race subgroup (β = 0.76 and 0.61, respectively, P = 0.02). In conclusion, 25(OH)D concentrations increased temporarily and then decreased during the 24 months following RYGB. The acute increase and the positive associations observed between adipose tissue mass and systemic 25(OH)D concentrations suggest storage in adipose tissue and release during weight loss</blockquote>.
  
 +The study discussed here: [[http://marshallprotocol.com/view_topic.php?id=8252&forum_id=39&jump_to=257136#p257136|Tea and coffee consumption in relation to vitamin D and calcium levels in Saudi adolescents]] provides additional evidence that non-supplemented populations have low 25D. 330 Saudi adolescents with average 25D levels of 8-10ng/mL.
 ===== References ===== ===== References =====
home/tests/25d.txt · Last modified: 09.14.2022 by 127.0.0.1
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